Hi,

I have one question. Before that let me explain my current work. Basically I am working on DNA Barcoding of sharks. I have one species of shark namely Cephaloscyliium sarawakensis. When I’ve done the sequencing and blast from the genbank I get result other species which is Cephaloscyllium umbratile. Both blast result either genbank and from BOLD gave me the same result. Maybe this is due to no record on DNA barcoding or other gene sequences/whole genome from C sarawakensis. That is why the blast result gave me the possible or nearest species. Even when I aligned my sequence with C umbratile, there was a few of nucleotide that different.

So my next plan to design a primer for C sarawakensis. Now my question is, since I don’t have any reference/whole genome from C sarawakensis, can I use the nearest species (in this case C umbratile) for my primer design?

This study is very important for me because there is a paper on the difficulties in identifying these genus. And maybe this is one of the reason mis-identification happened to C umbratile and somehow the result from the genbank/BOLD lead to different species and unfortunately no photos available for C. umbratile from BOLD or even paper cited in the genbank.