I need to develop ELISA method for plasma and organ homogenate samples of drug. However, I don't have blank plasma sample to construct the calibration curve. Can I construct the calibration based on water drug standards?
Dear colleague!
I agree with previous answer. Your standart solution depends of drug which you plan to test. You should to use whole plasma or HSA since majority of drugs interacts with this proteins. Please, pay attention to the fact, what plasma could be some heterogenic and give different signal in calibration curve. Try to do calibration curve as with plasma and HSA and compare them.
Yes, you should try to obtain negative plasma samples (ideally a pool of them) to construct your standard curve.
If its not possible at all, I would try to use PBS+HSA to simulate plasma. I would never use water.
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