I want to estimate 2C-value using leaves tissues that have been frozen in liquid nitrogen and stored at -80 ºC from two months ago. Can I grind these tissues in liquid nitrogen and then run them on a flow cytometer?
Frozen mouse tissues have already been used for the purpose of flow cytometry. For freezing the tumor tissues either cryopreservation of cell suspension or snap-freezing of tissue blocks can be done.
You can get the protocols by following the link below:-
http://onlinelibrary.wiley.com/doi/10.1002/cyto.990130708/pdf
Frozen mouse tissues have already been used for the purpose of flow cytometry. For freezing the tumor tissues either cryopreservation of cell suspension or snap-freezing of tissue blocks can be done.
You can get the protocols by following the link below:-
http://onlinelibrary.wiley.com/doi/10.1002/cyto.990130708/pdf
Do it possible to do cell surface staining and then run flow cytometry of the frozen human tumor tissue samples? Thanks.
I agree with Weihua Tian.
I haven't try frozen plant material to estimate 2C-value. But as I know, because of the presence of cell wall, nuclei isolation from plant tissue is quite more difficult than animal tissue. Maybe you can try some buffer. I would like to know if it works.
Best wishes.
Dear Dr. Pourkhaloee
Yes, it is possible but analysis of samples from frozen tissues usually results in lower resolution of histograms of DNA content and excessive background from debris.
Best wishes
Dear Dr. Pourkhaloee
Please have a look these papers
Article Flow cytometry in plant breeding
https://www.nature.com/articles/nprot.2007.310
Best wishes
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