Which software is the best for calculating polymorphic bands?

I assessed genetic diversity of 36 populations (288 individuals/8 individuals per population) using 8 NBS marker-restriction enzyme combinations and obtained 512 loci. Now, I want to calculate No....

09 October 2017 4,229 4 View

What are the advantages of recurrent somatic embryogenesis?

For example, secondary somatic embryogenesis has great potential for large scale micropropagation, which is especially important for those woody plants having long generation cycles, and with low...

10 November 2015 1,146 7 View

Can frozen tissues be used as samples for flow cytometry?

05 June 2015 5,981 8 View

In the realm of plant breeding, how can we distinguish among methods, techniques and strategies?

What are the exact differences among these three words?

04 May 2015 4,481 2 View

What is the best definition of cytogenetics and karyology?

What are the differences between cytology, cytogenetics, and karyology? How do they deal with each other?

02 March 2015 4,467 4 View

Are superabsorbent polymers autoclavable?

I want to use a super absorbent polymer in aseptic conditions.

01 February 2015 5,393 4 View

Is it possible to estimate the inheritance of a specific plant gene by new molecular methods prior to crossing?

Suppose we have located a specific gene on its related chromosome and will subsequently want to determine the inheritance of this gene before crossing.

01 February 2015 4,128 8 View

What are the differences between resistance and tolerance with regard to biotic and abiotic stresses in plants?

In some cases they are used alternatively. What is the best definition of each one?

01 February 2015 9,695 15 View

Does the turnover of plant extract sugars in refrigerator cause error during their measurement?

With an emphasis on the impact of low temperature on sugars and carbohydrates.

11 December 2014 4,723 9 View

Is it possible to assay the amount of sugars and carbohydrates in samples that have been kept in an ultra-low temperature freezer for one month?

The samples are frozen petals and leaves that have been frozen in liquid nitrogen and then kept in an ultra-low temperature freezer for one month.

11 December 2014 9,043 7 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

No title

03 March 2021 5,360 2 View

Why am I not able to use all VEP programs for the in silico analysis of avpr1b SNP's?

Good day researchers I am busy analysing the predicted effects of certain SNP's in AVPR1b. When using the VEP prediction programs (SIFT, Polyphen, FATHMM, LRT, Provean, Mutation Assessor, and...

28 February 2021 5,197 1 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View

What is the best way to calculate the angles of twist for concrete columns subjected to torsional moment using finite element software?

In Finite element software like ANSYS , there is difficulty in obtaining direct results of twisting of concrete members because SOLID65 which is the unique element designed to represent concrete...

26 February 2021 3,806 2 View

Compute for the total volume of concrete needed for the concrete pouring of the foundation including its column up to NGL only as shown in the plan?

Looking for the quantity of cement, sand, and gravel of footings and columns. Thank you!

25 February 2021 3,092 2 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Can I use TCA instead of TFA for protein analyis in RP-HPLC?

I am trying to analyze lipase using the reverse phase-HPLC C18 column. In the literature, 0.1 % TFA was and 60% ACN was used. Can I use TCA in place of TFA? If any other methods are available to...

25 February 2021 2,388 3 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View