For example, secondary somatic embryogenesis has great potential for large scale micropropagation, which is especially important for those woody plants having long generation cycles, and with low frequencies of somatic embryogenesis.
Recurrent embryogenesis means production of new somatic embryos from old germinating somatic embryos. This process not only helps in rapid multiplication but it is useful for genetic transformation research as well.
Recurrent embryogenesis enables a rapid multiplication of embryos, for large scale micropropagation, generating seedlings for diverse applications, such as: genetic transformation, germplasm conservation, production of pathogen-free seedlings, among others. However, it is important to emphasize that somaclonal variation still remains as a barrier for the conservation of genetic identity of a genotype. If you are interested in clonal propagation of a species, the direct rooting of apical meristems could represent a solution for this problem, since it avoids the dedifferentiation provoked by the callus cultures.
In woody plants having long generation cycles, we must consider that the use clonal propagation to produce selected materials could decrease dramatically essential genetic variability for resilience.
Rapid multiplication to reach the target figures at an early date . But if the number of subcultures cross 10 or more the quality of the propagules will decrease. Somaclonal variation can occur .You need to start fresh initiation when ever it happens
This method enables the production of great number of somatic embryos that can be utilized in artificial seed technology, gene transfer and cellular developmental biology. Direct recurrent somatic embryos also complete life cycle quickly with no callus phase , and could be of high genetic stability
Advantages of recurrent somatic embryogenesis include high multiplication rate, independence of an explant source and repeatability
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