The samples are frozen petals and leaves that have been frozen in liquid nitrogen and then kept in an ultra-low temperature freezer for one month.
Yes ! please refer JB Harbourne's Phytochemical Analysis book for further details for quantification of both. And yes if you stored at -80 C, or say -20 C , both soluble and insoluble polymeric sugars would be intact ! best wishes..
Thank you very much Dear Dr. Biswapriya Biswavas Misra.
I Appreciate the attention given.
If your samples have been stored at -80ºC for that time should be in good condition for analysis. I suggest prior to your analysis the use of liquid nitrogen to grind your samples and then extract your carbohydrates.
Regards.
Its definitely possible. If you are doing total sugar quantification for example by acid hydrolysis, there is absolutely no need for any kind of pretreatments.
Yes, it is possible. You could use this material for quantification with the Dubois et al. (1956) method after grinding in liquid nitrogen.
Best regards.
Yes, no problem. I suggest lyophilized samples so you can keep them for longer without keeping them in ultra-low-temperature (I hope you understand my english)
(:
Thank you very much Dear Dr. Aarón Barraza, Ms. Gayathri Murukesan, Mr. Yugo Lima‐Melo, and Dr. Martha Elva Ibarra-Estrada.
I Appreciate the attention given.
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