Danny Gazali

4 Questions 11 Answers 0 Followers

Questions related from Danny Gazali

How to dock immunological synapse protein complex?

Dear researchersI have to reconstruct the synapse from available PDB file. The file includes MHC/endogenous peptide/TCR, but in my case there are certain condition of sequences and/or structures...

04 April 2017 5,158 1 View

Any advices on how to perform qRT-PCR of rearrangement gene for sequencing?

Dear all I was trying to determine T-cell receptor (TCR) variant by sequencing from qRT-PCR product. Of qRT-PCR, I had optimised template concentration, annealing temp. and gave result with ΔCt of...

12 December 2015 3,623 2 View

Can we purify plasmid from cultures grown in rich media?

Hi there, In term of plasmid isolation, I tried to thrive DH5α host cell containing recombinant gene in three types of broth media which are LB, SOB, and SOC. All media added with appropriate...

01 January 2015 9,982 8 View

Does anyone have these scientific articles?

Hi there I am studying mechanism of two different plasmid type. Both articles seem fundamental to commence with. 1) Differential Replication of Plasmids during Stringent and Relaxed Response of...

11 November 2014 8,406 0 View