How to prevent getting fragmented villi structure in HE staining of small intestine sections?

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Robson Campos Gutierre

Dear Sailesh

Did you make any chemical fixation?

How did you make the dissection?

Sailesh Palikhe

Dear Robson Campos Gutierre,

After washing the intestine with PBS, I fixed the intestine by keeping it in 10% formaldehyde neutral buffer solution for overnight.

Kakhaber Zaalishvili

why did you use OCT? if you fixed the tissue in formalin, then you had to proceed with FFPE preparation. OCT and formalin are not compatible. if you need to preserve villi, make FFPE .