Articles published in journals contains both reference and citation, while books contain only reference. Does anybody have idea about it?
I am trying to perform HE staining of small intestine. I used OCT solution to make sections. After HE staining when I look my slides under microscope, I do not get intact villi. In much of the...
01 February 2020 5,792 3 View
We are currently working on a cytosolic protein; however, we think that this protein also has a secreted form and can be detected in the bloodstream. As there is no ELISA kit available for this...
01 February 2020 302 2 View
I want to study whether two proteins: Myc-tagged protein A and Flag-tagged protein B, interacts with each other or not. For that I co-expressed these proteins in HEK293T cells, and later (after 48...
01 February 2019 1,783 4 View
I want to determine whether protein A and protein B interacts with each other by co-immunoprecipitation analysis. For that I prepared Myc-tagged protein A and Flag-tagged protein B plasmid DNAs. I...
01 February 2019 844 4 View
I am just wondering whether different labs prepare immortalized bone marrow derived macrophages (BMDM) in their labs. I have experience of preparing primary BMDM from mice femur, but have no idea...
31 December 2018 8,803 2 View
I have been working on two proteins. And I think that these two proteins interact with each other. To prove that, I analyzed there association by endogenous IP, but unfortunately I could not get...
11 December 2018 9,618 4 View
I just started to do EMSA to check interaction of a protein and DNA. When I checked the binding buffer used for EMSA, I found that EDTA is also used. I know that EDTA is a chelating agent, and...
04 May 2018 2,540 1 View
I have been recently trying to isolate primary microglia from mice pups brain (P0-P1). To prepare mixed microglia culture, I used 3 brains for a 75 cm2 flask. After around 10-12 days, astrocyte...
03 April 2018 3,654 12 View
Currently, I am doing knockdown of certain genes by shRNA technique. We use lenti-virus for this process. The problem is that we are getting low titre. The experimental procedure is: Day 1: Seed 7...
04 May 2017 2,303 6 View
I am culturing an adherent cell in a 6-well plate. Unfortunately, one of the well got contaminated. Now, I need to change the media, and I just afraid while doing so the contamination get into...
08 September 2016 7,725 6 View
Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.
03 March 2021 7,174 5 View
Any suggestion on what book to read is also welcome.
03 March 2021 1,646 4 View
I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...
03 March 2021 8,920 3 View
How to upload pdf of my articles published?
03 March 2021 2,183 3 View
Anyone can recommend any books on such topics? Thanks in advance.
02 March 2021 8,457 2 View
I have an issue with APA 7th in-text citation. II have two papers where the first three authors are the same, and the fourth is different. I am using the Mendeley desktop app, and automatically it...
02 March 2021 790 3 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...
02 March 2021 8,437 2 View
Please provide Book Title and author name
02 March 2021 9,059 3 View
I would like to know how to register the journal Flora Montiberica (ISSN 1138-5952 - eISSN 1988-799X) in Research Gate.
02 March 2021 8,156 2 View