Articles published in journals contains both reference and citation, while books contain only reference. Does anybody have idea about it?
I am trying to perform HE staining of small intestine. I used OCT solution to make sections. After HE staining when I look my slides under microscope, I do not get intact villi. In much of the...
01 February 2020 5,960 3 View
We are currently working on a cytosolic protein; however, we think that this protein also has a secreted form and can be detected in the bloodstream. As there is no ELISA kit available for this...
01 February 2020 468 2 View
I want to study whether two proteins: Myc-tagged protein A and Flag-tagged protein B, interacts with each other or not. For that I co-expressed these proteins in HEK293T cells, and later (after 48...
01 February 2019 1,927 4 View
I want to determine whether protein A and protein B interacts with each other by co-immunoprecipitation analysis. For that I prepared Myc-tagged protein A and Flag-tagged protein B plasmid DNAs. I...
01 February 2019 994 4 View
I am just wondering whether different labs prepare immortalized bone marrow derived macrophages (BMDM) in their labs. I have experience of preparing primary BMDM from mice femur, but have no idea...
31 December 2018 8,956 2 View
I have been working on two proteins. And I think that these two proteins interact with each other. To prove that, I analyzed there association by endogenous IP, but unfortunately I could not get...
11 December 2018 9,770 4 View
I just started to do EMSA to check interaction of a protein and DNA. When I checked the binding buffer used for EMSA, I found that EDTA is also used. I know that EDTA is a chelating agent, and...
04 May 2018 2,719 1 View
I have been recently trying to isolate primary microglia from mice pups brain (P0-P1). To prepare mixed microglia culture, I used 3 brains for a 75 cm2 flask. After around 10-12 days, astrocyte...
03 April 2018 3,801 12 View
Currently, I am doing knockdown of certain genes by shRNA technique. We use lenti-virus for this process. The problem is that we are getting low titre. The experimental procedure is: Day 1: Seed 7...
04 May 2017 2,452 6 View
I am culturing an adherent cell in a 6-well plate. Unfortunately, one of the well got contaminated. Now, I need to change the media, and I just afraid while doing so the contamination get into...
08 September 2016 7,925 6 View
in the text it is cited in square bracket. in the reference list, it is in the order; author/s, title, journal, year, issue(volume), page. or DOI eg. Smith JJ. The world of...
09 August 2024 9,355 1 View
How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...
08 August 2024 6,690 3 View
Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...
07 August 2024 9,563 0 View
I am Looking for a Science Journal with good impact factor and low publication cost to publish a review paper. Your suggestions would be appreciated.
06 August 2024 6,796 3 View
I received an e-mail invitation to join the editorial board of Clinical Cardiology Updates. While I have published a few articles related to cardiovascular disease, there are lots of colleagues...
06 August 2024 8,981 8 View
If the placenta is delivered in squatting position within 3 minutes of the birth of the baby, postpartum hemorrhage is completely eliminated. It works every time. It is quite likely that if a...
05 August 2024 8,895 0 View
Hello everyone I am looking for one or some books for propertes and behaviors of stochastic fibre orientation composites. unfotunately I could not find any suitable reference for thias by...
04 August 2024 3,461 3 View
How to enhanced h-index in Research Gate?
04 August 2024 3,368 4 View
As authors and academic writers, we usually prepare our manuscript using our own resources. We submit these manuscripts to scientific Journals for peer review and publication sometimes at a fee....
03 August 2024 7,304 2 View
need to open an account to upload my published papers
01 August 2024 9,255 1 View