Claudia Machicado

12 Questions 13 Answers 0 Followers

Questions related from Claudia Machicado

Identification of bacterial enzymes from human transcriptome data?

Hello, Do you know if is that possible to identify bacterial enzymes (from microbiota) by analyzing transcriptomics data from a human tissue (i.e. TCGA samples)? Thanks for you response!

17 February 2023 5,720 3 View

Protein digestion by a given protease?

I have a given protease (with known sequence and structure). Is that possible to predict targets (with known sequence) through bioinformatics approaches?

20 June 2022 1,001 1 View

How to determine if my sequences appear in a given transcriptome?

Hi RG community, We have more than 4,000 protein sequences that we want to cross against a given transcriptome, to determine if these appear in such transcriptome. How to do that?

11 January 2021 1,806 4 View

Reasons for Covid-19 false positives in pediatric cancer?

Hello, Any idea why false positives appear in children with cancer (neuroblastoma, osteosarcoma, etc) under chemotherapy? Rapid tests result positive but PCR results negative. Thanks,

18 June 2020 3,453 3 View

How identifying metabolites in NMR spectra?

Hi, I have the raw data (chemical shifts and intensities) obtained from NMR experiment in blood samples. How can we recognize the metabolites and concentration present per participant? We have...

02 September 2019 7,210 6 View

Should I analyze standards before a GC/MS assay?

I need to identify four compounds (VOCs) from a MS spectrum including propionic acid, valeric acid, etc. Should I utilize standards before my run? Or there is a database we can utilize to...

06 November 2018 5,283 12 View

How to preserve a fresh tissue after surgery for future molecular analysis (cancer research)?

After surgery, what is the best condition to preserve fresh tissue samples from cancer patients for future molecular analysis? For instance: glycerol, formol, saline buffer? We will analyze these...

27 October 2016 7,042 4 View

Any bioinformatic tool or program suitable to find binding sites in proteins?

I need to find out if my protein has binding sites available for intermolecular interaction with other proteins.

29 September 2016 8,768 3 View

How to study cell membranes from FFPE samples?

Which is the best method to study cell membranes (lipids and proteins) from FFPE samples?

29 April 2016 5,960 0 View

Can deparaffinization damage cell membranes?

Anyone knows if the procedure of deparaffinization can alter the structure/composition of plasma membranes?

28 April 2016 9,531 6 View

How to compute electrostatic potential by using commercial bioinformatic software?

I'm trying to compute and represent the electrostatic potential (Poisson-Boltzmann) of some proteins to verify if they can potentially bind in their complementary interfaces. Is there any...

08 April 2016 7,418 5 View

Is there anyone familiarized with clouds in biomedical research? Or any publication I could review for guidance?

Big Data Analysis

05 March 2016 8,358 3 View