Can cytochrome C reduction by complex III be assayed without the addition of ubiquinol to reaction mixture?

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I'm not able to see the overexpression of my antitoxin on SDS PAGE of my chosen toxin-antitoxin system of mycobacterial species. Is there anything ?

I have been trying to check the overexpression of the antitoxin of my chosen TA system of mycobacterium sp. I have tried to induce the clone at 20, 25 and 37° C at 0.1, 1 and 10 mM concentration...

22 May 2024 1,334 0 View

I'm not able to purify my protein, is it due to the column or the protocol?

I'm using Ni-NTA resin (Qiagen) column to purify my his-tagged protein. Over a period of time the color of my column has changed to color less. I'm using the concentration you can see in the...

15 May 2024 2,573 4 View

Why my NiSO4(H2O)6 is not dissolving in 1X PBS buffer solution?

I am trying to recharge the Ni-NTA column for his-tagged protein purification but my NiSO4(H2O)6 is not dissolving in 1x PBS and I have tried it from a different reagent bottle also, please help!

15 May 2024 9,746 3 View

How tourism industry can become a hub for providing employment to the masses?

Tourism industry is a big service provider and lot of scope for generating different types of employment

15 August 2023 8,271 2 View

What is the level of measurement and correlation to be used in SPSS for these two scales?

Hi everyone, I'm a newbie here and to writing researches as well. I'm currently engaged in writing my first research (dissertation) for academic purposes and need some help with the same. Here...

29 April 2023 844 1 View

May I have recommendations for labs/institutes that can conduct in-vitro and/or in-vivo test on covid strain?

Hi We have developed a set of 2 plant extract based oils. Topical application of the oils have shown efficacy against RNA enveloped family of viruses as well as on 1100+ covid symptomatic...

21 July 2020 1,652 0 View

Is there a way to keep nickel phosphate from precipitating when nickel chloride is added top PBS?

I need to add NiCl2 to PBS for an assay but it causes turbidity, possible due to precipitates of nickel phosphate. is there a way to prevent this?

20 July 2016 7,431 1 View

How much lipoxygenase activity do MCF -7 cells have?

I am testing MCF -7 cells for lipoxygenasev activity but cant find any literature that reports the specific activity that these cell have. Any input will be appreciated. Thanks

29 June 2016 842 2 View

Does the detergent NP-40 interfere with Berthelot test for ammonia?

I am running an experiment to detect ammonia produced in a biological system die to the action of an enzyme using the Berthelot reaction. It seems like the addition of detergent NP-40 to my...

21 June 2016 9,045 2 View

How long doe MCF-7 cells take to attach after thawing and putting in a flask?

I am growing MCF-7 cells and after 4 hours of thawing and putting in a flask, the cells are still floating. I am worried because this is an adherent cell line and should not take this long to...

17 June 2016 4,308 3 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

Does anyone know any C++ implementation of Kolmogorov-Arnold network other than mine?

Mine is sitting here: http://openkan.org/DLpiecewiseCPP.html I wish to see someone else's.

06 July 2024 9,974 1 View

Which is better for the student : Implementing the principles of object-oriented programming using Java or C++?

Object-Oriented Programming

29 June 2024 4,877 12 View

How can I better show my data?

I am validating the expression of several genes by qPCR to confirm transcriptome analysis. After many inconclusive runs, I realised my housekeeping gene (GAPDH) was downregulated in my...

27 June 2024 7,318 3 View

Any specific peptide based ligand for mitochondrial targeted delivery of nano-drug delivery systems?

Suppose I need to target mitochondria to address malignancy-related problems. Are there peptide-based ligands similar to Nuclear Localization Signals (NLS) for nuclear targeting that can be used...

20 June 2024 9,587 1 View

What is the exact mathematical formulae and the C++ code of EMMS drag model that is used and applied in ANSYS Fluent 2023?

EMMS - Energy Minimization Multi-Scale

18 June 2024 5,857 2 View

How to enumerate mitochondria? and how to isolate them from a single cell suspension?

I am interested in analyzing the effects of some immunotherapy on the number of mitochondria in cancer cells as well as in immune cells. I would like also to isolate mitochondria to use them for...

16 June 2024 9,632 1 View

Staining of isolated mitochondria tecnique?

Greetings, dear colleagues! Do you know any technique utilizing some ~ordinary molbiol lab reagents (not the commertial kits for isolated mitochondria staining) that will allow isolated...

07 June 2024 5,909 0 View

Histology - mitochondria: Hi RG members. Is anybody aware whether a reliable method for determining mitochondrial density exists for light microscopy?

I would like to look at mitochondrial density in transverse sections of intestine. I do not have access to a TEM. I cannot really determine based on an internet search whether it is feasible to do...

02 June 2024 5,373 2 View

"How can federated learning and edge computing architectures be designed to ensure compliance with GDPR and HIPAA regulations ?

Designing Federated Learning and Edge Computing Architectures for GDPR and HIPAA Compliance in Healthcare Applications.

28 May 2024 6,525 1 View