How tourism industry can become a hub for providing employment to the masses?

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What are the limitations and challenges of using machine learning for predicting concrete compressive strength in practical applications?

Machine learning (ML) has shown great potential in predicting the compressive strength of concrete, an important property for structural engineering. However, its practical application comes with...

03 August 2024 2,546 2 View

I'm not able to see the overexpression of my antitoxin on SDS PAGE of my chosen toxin-antitoxin system of mycobacterial species. Is there anything ?

I have been trying to check the overexpression of the antitoxin of my chosen TA system of mycobacterium sp. I have tried to induce the clone at 20, 25 and 37° C at 0.1, 1 and 10 mM concentration...

22 May 2024 1,334 0 View

I'm not able to purify my protein, is it due to the column or the protocol?

I'm using Ni-NTA resin (Qiagen) column to purify my his-tagged protein. Over a period of time the color of my column has changed to color less. I'm using the concentration you can see in the...

15 May 2024 2,573 4 View

Why my NiSO4(H2O)6 is not dissolving in 1X PBS buffer solution?

I am trying to recharge the Ni-NTA column for his-tagged protein purification but my NiSO4(H2O)6 is not dissolving in 1x PBS and I have tried it from a different reagent bottle also, please help!

15 May 2024 9,746 3 View

How to quantify Short Chain Fatty Acid in Stool samples of mice?

I am doing experiments in mice model of metabolic disorder. For that I have to do quantification od 3 major short chain fatty acid namely acetate propionate and butyrate. I had earlier tried...

15 April 2024 3,284 0 View

Can Climate Change and Earthquake are interconnected?

Increasing rate of Earthquakes.

04 April 2024 6,766 3 View

FOLD CHANGE calculation for metabolomics data?

I have metabolomics data from a non-targeted approach normalized with internal standards. I have calculated the p-value (t-test), VIP score, and fold change. For that, I transformed the data (each...

23 March 2024 9,663 1 View

Untreated cells are showing high LDH reading than treated cells?

I did LDH cytotoxicity assay on HCT116 cell line with my compounds at various concentrations. After 24,48,72h of incubation, LDH assay was performed according to manufacturer's protocol. My...

10 March 2024 8,521 0 View

I want to test the following hypothesis using Mann -Whitney U Test. Is my interpretation of the result correct?

Null Hypothesis: The recovery percentage of A channel is less than the recovery percentage of B channel Alternate hypothesis: The recovery percentage of A channel is not less than the recovery...

02 March 2024 4,903 4 View

Can anyone suggest how to write results of Kruskal wallis test in thesis?

The following are the results of 4 channels of recovery of NPAs H =21.402153 Chi square value= 7.814727903 , Alpha level= 0.05 P value 0.00008685210 Reject Null? Reject Null

23 February 2024 6,835 5 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Where to find a gene list for CRISPRa/i library screening of regulatory factors that affect pathogenic Th17 differentiation in PBMC?

I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...

31 July 2024 2,150 0 View

Hello dear researchers. I would like to know what is a normalized mass in an EDX analysis?

EDX analisys

31 July 2024 5,947 2 View

Gas chromatography RT detection?

I am working on algal extract to which gas chromatography (Not GC-MS) spectrum I want to discover. My question is can we identify specific compounds using retention time if I compared the RT with...

29 July 2024 8,034 4 View

MAGeCK Plotting Error: Logarithmic Axis Must Have Positive Limits?

I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...

28 July 2024 9,811 1 View

Screening of EPS producer LAB?

if you have MRS broth already prepared, what is the manner you will follow for preparing hyper-sweet medium for screening of EPS producer LAB? what the concentration of saccharose or glucose you...

26 July 2024 6,606 0 View

Could international experts in media and journalism, AI, communications and international communication share this conference link?

"Integration of Mass Media and Communication into the System of International Relations: Place, Role, Actuality and Prospects: Modern Media Issues 2024" Website: https://immcir.alnoor.edu.iq/

23 July 2024 3,139 6 View

Why methanol and sulphuric acid used in the analysis of polyhydroxyalkanoates (PHA) by GC-MS?

Why methanol and sulphuric acid, used in the analysis of polyhydroxyalkanoates (PHA) methyl esters by GC-MS? Additionally, why do we typically use non-polar solvents in GC-MS?

22 July 2024 1,210 2 View