How to correct background in Ce3d XPS data using CASAXPS software?

I have high-resolution XPS data of Ce3d (attached) in which Shirley background is crossing my actual data as shown in the figure. I need to correct it. Please find the attached '.txt' data file....

23 January 2020 2,535 6 View

What kind of properties are needed in ANSYS or other FEA packages to conduct the stress/strain analysis of 2D materials under tension, and bending?

I want to simulate the effect of stresses on a graphene monolayer and observe the deformations appearing in the material. The problem is the understanding of the element size and how to define a...

02 August 2019 9,005 4 View

Which type of filter membrane would be useful for pre-treatment of urine prior to ION CHROMATOGRAPHY analysis?

A standard protocol for pre-treatment of urine prior to analysis is required.

31 May 2019 7,386 3 View

What are some high level international conferences in which scientists have discussed about threats posed by microbes such as Candida, staph, etc ?

I am searching for some high level international scientific conferences in which world's top scientists have discussed about the serious diseases caused by the microbes found on our skin, head and...

04 February 2019 8,167 6 View

How to design a robust PID controller for a system with parametric uncertainties? How to implement it in MATLAB?

How can I design a robust PID controller for a second order plant having parametric uncertainties using h infinity. I tried using 'hinfsyn' function in matlab but it gives a higher order...

29 June 2018 942 5 View

How to keep size of the seed different for different regions in abaqus?

I want the width of the seed in the upper and lower sections to be different(more) than that of the seed in the middle region. I have specified different seed sizes for the edges in the middle and...

25 September 2016 2,444 11 View

What should be the plastic strain, when defining yield stress in Abaqus?

Isn"t the plastic strain zero at yield stress(atleast for non-brittle metals)?

03 September 2016 7,023 21 View

How long doe MCF-7 cells take to attach after thawing and putting in a flask?

I am growing MCF-7 cells and after 4 hours of thawing and putting in a flask, the cells are still floating. I am worried because this is an adherent cell line and should not take this long to...

17 June 2016 4,239 3 View

Is it better to run complex III activity assay without sodium azide?

I am running an assay for activity of mitochondrial complex III. Most protocols use sodium azide / KCN as an inhibitor of complex IV to prevent re oxidation on cytochrome c. However, on addition...

07 March 2016 848 1 View

Do I need to add sodium azide/potassium cyanide and antimycin A while checking for complex 1 enzyme activity?

03 March 2016 382 4 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Why tick's salivary gland extract didn't run in the gel (SDS PAGE )??

Greetings, I want to make SDS PAGE for my sample (Tick salivery gland extract), I tried concentrations 10%, 12%. I also tried high molecular weight ladder. But the protein stopped at the top and...

25 February 2021 7,698 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View