16 May 2024 3 10K Report

I am trying to recharge the Ni-NTA column for his-tagged protein purification but my NiSO4(H2O)6 is not dissolving in 1x PBS and I have tried it from a different reagent bottle also, please help!

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I'm not able to see the overexpression of my antitoxin on SDS PAGE of my chosen toxin-antitoxin system of mycobacterial species. Is there anything ?

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I'm not able to purify my protein, is it due to the column or the protocol?

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15 May 2024 2,573 4 View

Can anyone tell me how to cite and reference a paper which correct citation is not available in Google Scholar or how to create a citation?

Or how to create a citation and reference using information available in a paper?

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How tourism industry can become a hub for providing employment to the masses?

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How to use profilometry or Ellipsometry to measure the thickness of spin coated PbS quantum dot films on CVD graphene on copper?

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Are there any appropriate polymers that can be used to encapsulate PbS quantum dots with 1-2 EDT ligands?

I have a stack of PbS quantum dots with 1-2 EDT ligands that I need to encapsulate. I have tried encapsulating it with PMMA and SU8 however it seems that the cross linking is being inhibited by...

02 July 2023 7,598 4 View

What is a good tool to cut thin copper foil with graphene (25um) into circles?

I have some CVD graphene on copper that I need to cut into circular discs. I can obviously do it using scissors but it wouldn't be super precise and can damage the film in surrounding areas. Would...

20 June 2023 3,543 4 View

What is Joule Thompson effect ?

I am not able to understand some basics. I require a detailed understanding and visual demonstration, as I am going to use it in my next research project.

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What is the level of measurement and correlation to be used in SPSS for these two scales?

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