How to correct background in Ce3d XPS data using CASAXPS software?

I have high-resolution XPS data of Ce3d (attached) in which Shirley background is crossing my actual data as shown in the figure. I need to correct it. Please find the attached '.txt' data file....

23 January 2020 2,535 6 View

What kind of properties are needed in ANSYS or other FEA packages to conduct the stress/strain analysis of 2D materials under tension, and bending?

I want to simulate the effect of stresses on a graphene monolayer and observe the deformations appearing in the material. The problem is the understanding of the element size and how to define a...

02 August 2019 9,005 4 View

Which type of filter membrane would be useful for pre-treatment of urine prior to ION CHROMATOGRAPHY analysis?

A standard protocol for pre-treatment of urine prior to analysis is required.

31 May 2019 7,386 3 View

What are some high level international conferences in which scientists have discussed about threats posed by microbes such as Candida, staph, etc ?

I am searching for some high level international scientific conferences in which world's top scientists have discussed about the serious diseases caused by the microbes found on our skin, head and...

04 February 2019 8,167 6 View

How to design a robust PID controller for a system with parametric uncertainties? How to implement it in MATLAB?

How can I design a robust PID controller for a second order plant having parametric uncertainties using h infinity. I tried using 'hinfsyn' function in matlab but it gives a higher order...

29 June 2018 942 5 View

How to keep size of the seed different for different regions in abaqus?

I want the width of the seed in the upper and lower sections to be different(more) than that of the seed in the middle region. I have specified different seed sizes for the edges in the middle and...

25 September 2016 2,444 11 View

What should be the plastic strain, when defining yield stress in Abaqus?

Isn"t the plastic strain zero at yield stress(atleast for non-brittle metals)?

03 September 2016 7,023 21 View

How long doe MCF-7 cells take to attach after thawing and putting in a flask?

I am growing MCF-7 cells and after 4 hours of thawing and putting in a flask, the cells are still floating. I am worried because this is an adherent cell line and should not take this long to...

17 June 2016 4,239 3 View

Is it better to run complex III activity assay without sodium azide?

I am running an assay for activity of mitochondrial complex III. Most protocols use sodium azide / KCN as an inhibitor of complex IV to prevent re oxidation on cytochrome c. However, on addition...

07 March 2016 848 1 View

Do I need to add sodium azide/potassium cyanide and antimycin A while checking for complex 1 enzyme activity?

03 March 2016 382 4 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View