How much lipoxygenase activity do MCF -7 cells have?

More Vasundhra Bahl's questions See All

I'm not able to see the overexpression of my antitoxin on SDS PAGE of my chosen toxin-antitoxin system of mycobacterial species. Is there anything ?

I have been trying to check the overexpression of the antitoxin of my chosen TA system of mycobacterium sp. I have tried to induce the clone at 20, 25 and 37° C at 0.1, 1 and 10 mM concentration...

22 May 2024 1,334 0 View

I'm not able to purify my protein, is it due to the column or the protocol?

I'm using Ni-NTA resin (Qiagen) column to purify my his-tagged protein. Over a period of time the color of my column has changed to color less. I'm using the concentration you can see in the...

15 May 2024 2,573 4 View

Why my NiSO4(H2O)6 is not dissolving in 1X PBS buffer solution?

I am trying to recharge the Ni-NTA column for his-tagged protein purification but my NiSO4(H2O)6 is not dissolving in 1x PBS and I have tried it from a different reagent bottle also, please help!

15 May 2024 9,746 3 View

How tourism industry can become a hub for providing employment to the masses?

Tourism industry is a big service provider and lot of scope for generating different types of employment

15 August 2023 8,271 2 View

What is the level of measurement and correlation to be used in SPSS for these two scales?

Hi everyone, I'm a newbie here and to writing researches as well. I'm currently engaged in writing my first research (dissertation) for academic purposes and need some help with the same. Here...

29 April 2023 844 1 View

May I have recommendations for labs/institutes that can conduct in-vitro and/or in-vivo test on covid strain?

Hi We have developed a set of 2 plant extract based oils. Topical application of the oils have shown efficacy against RNA enveloped family of viruses as well as on 1100+ covid symptomatic...

21 July 2020 1,652 0 View

Is there a way to keep nickel phosphate from precipitating when nickel chloride is added top PBS?

I need to add NiCl2 to PBS for an assay but it causes turbidity, possible due to precipitates of nickel phosphate. is there a way to prevent this?

20 July 2016 7,431 1 View

Does the detergent NP-40 interfere with Berthelot test for ammonia?

I am running an experiment to detect ammonia produced in a biological system die to the action of an enzyme using the Berthelot reaction. It seems like the addition of detergent NP-40 to my...

21 June 2016 9,045 2 View

How long doe MCF-7 cells take to attach after thawing and putting in a flask?

I am growing MCF-7 cells and after 4 hours of thawing and putting in a flask, the cells are still floating. I am worried because this is an adherent cell line and should not take this long to...

17 June 2016 4,308 3 View

How can i perform accelerated stability calculations for cytochrome c and NADH?

I need to perform accelerated stability calculations for cytochrome c and NADH. I have lyophilized samples stored at different temperatures for different time. How can I obtain the percent...

03 May 2016 1,319 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View