Can competent E coli cells be stored safely at -125C?

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Valeria De Arcangelis

Hi, I agrre with the previous answer if you have "handmade" competent cells. If you have also "buyed" competent cells, I don't think it is a good idea. Some companies do not recommend temperature different than -80

Gopal Tiwari

Yes you can

William F Sutton

They are shipped on dry ice. My inclination is to say yes, storable at -125C.

Christian Q. Scheckhuber

I agree, no problem in storing the cells at -125°C (might be even better than -80°C!).

Cheers, Christian