Gopal Tiwari

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Questions related from Gopal Tiwari

Is there any relation between protein concentration and activity band intensity in zymogram?

When I run SDS PAGE gel and perform zymogram with same gel.I observed that the bands which are more intense (more protein) after CBB staining give less intense activity bands in zymogram, whereas...

26 June 2015 2,444 6 View

Does glycosylated protein shows different band size on nitrocellulose and PVDF membrane??

I expressed protein in P.pastoris and performed zymogram (to check enzyme activity in gel) and did western blotting using Nitrocellulose membrane, in both band observed was in same position...

27 May 2015 8,630 3 View