No smears on gel after cDNA synthesis ?

Hi all, I have a problem with detection of cDNA synthesis where when I put it on the gel , there were any smears so what the meaning of that and what can I do? Thanks

21 December 2020 1,126 2 View

Is e.coli ok at Electroporation at 30C?

For reference: I am transforming a newly assembled plasmid what happened: I accidentally incubated my electroporated e.coli cells on the shaker at 30C for 1 hr rather than 37C and did not...

02 December 2020 7,133 5 View

How to properly collect/store exosomes (isolated from conditioned culture media) for a later proteomic and/or metabolomic profiling?

I want to ask how to properly collect and store exosomes just after their isolation from conditioned culture media, for subsequent proteomic and/or metabolomic profiling ?? Should we make snap...

06 November 2020 5,201 3 View

How to develop a framework/model and present it in an analytical form?

Dear All, I am conducting a study on DBD actuators and I'm trying to develop a framework for actuators using plasma-based metamaterials. In order to reach this objective, I need to learn how to...

01 June 2020 3,864 2 View

Duplex PCR Target A & IC & target B & Ic works great. When multiplex the three together A FAM , B HEX, IC Cy5 , A & IC fine, B curve plateau strange?

The curve by B in Hex starts great but the plateau looks like zigzag. I tested different primer concentrations and different annealing temperatures. We use Lightcycler 480 II. Color compensation...

10 May 2020 7,879 3 View

Are ACEIs underdeveloped in children?

COVID19 shows less affection of lung in children . this may be due to underdeveloped or decreased expression of ACEI in lungs of children taking in consideration that as age increase from 5...

28 March 2020 2,448 2 View

Solution not converged at time ........... in ansys?

I am doing four point flexural text on simply supported beam using ANSYS APDL first with steel plate for loading and supporting and then without it. In both cases there is a problem of non...

19 March 2020 4,775 6 View

Can we find the Link between Teaching Methods and Achievement in Math in Computer-Assisted Elementary Schools?

I published a paper in: Creative Education, Published Online http://www.scirp.org/journal/PaperInformation.aspx?PaperID=47205 The Link between Teaching Methods and Achievement in Math in...

14 February 2020 1,408 4 View

How to detrend a series in Stata when it has a stochastic trend?

08 February 2020 1,803 3 View

What is the editorial office email of this journal?

I submitted an article and received an email stating that I need to fix the word count to 5000 or less., though the word count in my manuscript is 4330. I sent back an email in this regard and...

06 October 2019 9,154 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

What role should journals play in leading academic fields?

A recent paper by Yeung and Thomas (2021) on journals in event management opens up an interesting debate on the role of journals as academic fora. They demonstrate that event management publishing...

28 February 2021 3,494 6 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

Where i will get the Desmodium and Napier grass seeds?

26 February 2021 975 4 View

What is recommended and opened real-time or nearly real time geospatial data used in flood risk assessment and regional planning on a detailed scale?

I asked recommended real-time or nearly real-time data source for any parameters for flood risk on a microscale analysis for urban area cases in developing countries that had agglomeration urban...

18 February 2021 2,617 2 View