Can bacteria growth on agarose?

More Manon Oudijn's questions See All

Should I conduct a reliability check with the informations that the factor analysis gave me?

Hello everyone :) For my study, I would like to conduct a factor analysis followed by a reliability test for one of my concepts "Attitude towards unemployed" measured according to 5 items with a...

09 July 2023 9,461 3 View

What is wrong with the Laser dissection when the catapulting doesn't work?

I want to use the laser dissection function of the Zeiss PALM microscope. We have PEN membrane Petri dishes and cultured cells on them. When i laser the cells i can see the laser and also the...

24 April 2023 8,682 2 View

How to compare multiple proportions in R?

I would like to compare how different species use their habitat. Habitat use is measured as the number of times an individual is observed inside the habitat, close to that habitat, or far from it...

31 January 2023 1,150 4 View

How to solubilize oily plant extracts in cell culture media in order to analyse the therapeutics effects (on keratinocytes/fibroblasts) ?

The oily preparations contain mostly oleic acid (around 40%) We tried : - DMSO, EtOh 70, 90%, mixing solvent with EtOh / Tween 20 / Water - With or without sonication step (RT and 50°C) We...

11 May 2022 3,405 0 View

Which gradient and ultracentrifugation settings are the most relevant to purify CVB3 and SARS-CoV 2?

Hello, I am looking to purify Coxsackievirus B3 (CVB3) and SARS-CoV 2 viruses by ultracentrifugation (Optima XPN from Beckman Coulter) and after several searches in the literature, I decided to...

25 November 2021 6,199 1 View

What antibody (& protocol) for an immunoprecipitation against PSD-95?

Hello everyone, I am currently setting up IP against the PSD-95 protein, unsuccessfully at the moment. Could you please tell me which PSD-95 antibody you are using for an IP? And what protocol...

22 April 2021 7,434 0 View

What antibody (& protocol) for an immunoprecipitation against HA?

Hello, I am currently setting up IP from cells overexpressing a protein tagged with HA. But I am not able to pull down my protein. Could you tell me which HA antibody you are using for an IP? And...

22 April 2021 1,805 3 View

What can be the sources of pain after a total pancreactomy in case of chronic genetic pancreatitis ?

My mother, aged 39, has chronic genetic pancreatitis since at least 15 years. She has known several episodes of severe pain and went through lots of surgery that were unsuccessfull. She had a...

09 April 2021 4,450 6 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 2,321 2 View

Which statistical model to use for samples taken over time?

Hello everyone, I am currently analyzing my dataset, I have obtained body weight data in insects for 7 days consecutively exposed to six different treatments in triplicates. I would like to...

13 October 2020 5,829 12 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

Help. How can I fix my T7 reaction problems?

I start with IDT ordered 124 bp ssDNA that is PCR amplified, then I use a QIAgen agarose gel extraction to get my exact product, and I'm trying to purify ~95bp RNA by denaturing PAGE to prevent...

16 July 2024 4,604 0 View

How much 1X TAE buffer should I use in preparing 1.2% agarose gel?

Commonly, we prepare 1.0% of agarose gel with 0.4g agarose powder and 40ml 1X TAE buffer. if I would like to prepare a 1.2%% of agarose gel, is it I need to add 0.48g agarose powder and 120ml 1X...

12 July 2024 2,058 5 View

How to improve my plaque assay for influenza virus?

I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...

11 July 2024 5,669 5 View

Can I use DNA a ladder for RNA samples in agarose electrophoresis? Or It is preferable to use cDNA amplified from RT-qPCR for the same samples?

I performed a nucleic acid extraction using 2 protocols for RNA extraction (Dengue Virus) and one parameter I need to evaluate is the integrity of my RNA samples. In this case, I can't use a RNA...

09 July 2024 4,857 2 View

What is the reason of stacking DNA in agarose gel well?

we electrophoresis PCR product on 0.7% agarose gel at 120V for 1 hour, our target size is 5000 bp but our DNA stack at wells and do not com in to gelldo you have any suggestion for this?

02 July 2024 5,235 0 View

How can I increase DNA concentration after gel purification for long length DNA fragment?

I'm currently working on gel purification in order to get DNA fragment for T4 ligation. But I have been able to take only 8.5ng/μl as a highest concentration of DNA fragment. And could not get...

01 July 2024 4,912 9 View

What could be causing these smears in my PCR electrophoresis gel?

I am new to running PCR gels. I loaded this gel and I thought it was fine, meaning I saw/felt no apparent punctures or spillovers to neighboring wells (see picture 1). When the gel started to run,...

30 June 2024 4,107 4 View

Why is my zebrafish sample falling out of the agarose embedding?

Hi all, I am looking for advice on what could be going wrong with my zebrafish caudal fin tissue fixation that would cause the sample to fall out of the embedding while cryosectioning. During cryo...

25 June 2024 4,602 0 View

Katie A S Burnette

Yes, most likely they will grow just fine.

The majority of the nutrients are provided by the other components in the medium.

Heck of an expensive mistake to make though! Agarose is about $1 per gram and agar-agar is maybe 10 cents a gram.

Manon Oudijn

Thanks for your answer! Yess i knoww so expensive. I have incubate it for a week and i don't see any bacterie on it....