How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Which statistical model to use for samples taken over time?

Hello everyone, I am currently analyzing my dataset, I have obtained body weight data in insects for 7 days consecutively exposed to six different treatments in triplicates. I would like to...

13 October 2020 5,668 12 View

How big does the sample size for Spearman need to be?

For my research I have two sample sizes; one consists of 195 cases and the other of 31. The rho of the 195 cases is much bigger then the rho for the 31 cases. I tried to search for the minimum...

08 January 2020 7,193 7 View

How to remove the maximum amount of RNAlater Solution when working with Drosophila gut tissue ?

Hello everyone, I would like to use RNAlater Solution to store Drosophila gut before extraction. I would like to do that because I have a lot of guts to dissect and I cannot put them directly in...

03 July 2019 6,384 4 View

What would be a good toolbox to analyze and visualize eye tracking data (fixations and saccades)?

I am looking for suitable toolbox/package to analyze the eye-tracking data, using R, Matlab or Python. We have a Tobii T60 system and eye tracking data is simultaneously recorded with EEG data. We...

19 February 2018 1,428 13 View

Could you help me identify some taxidermy ?

26 May 2016 3,909 16 View

What is the sequence of the Neomycin Resistance cassette in Hek293T cells?

There have been numerous posts on this site related to neomycin resistance conferred by Hek293T's T-antigen...

22 September 2015 5,799 2 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View

Tapestation vs Agarose gel following PCR reaction - what are the extra bands?

We recently have been moving away from using agarose gels and onto using the Tapestation (D1000 for PCR products). However, we have noticed that there are a lot of extra bands that show up on the...

15 February 2021 791 3 View

Which distance should I measure to calculate the voltage to use for my agarose gel electrophoresis?

Hi, I've been reading recommendations of which voltage to use for agarose gel electrophoresis and they are often given in V/cm, where the cm refers to the "distance between the anode and cathode"...

14 February 2021 6,463 3 View

How long can agarose embedded tissues be kept?

I am embedding PFA fixed tissues in low melting point agarose prior to sectioning and I am wondering how long can these can be kept whilst maintaining tissue integrity and fluorescent signal?...

14 February 2021 6,688 3 View

Why does the band sizes on Agarose gel electrophoresis differ from the peaks at capillary electrophoresis?

14 February 2021 4,773 4 View

How much should I centrifuge cells in agarose-coated plates to get spheroids?

Right now I am using HT29 cells, but I will also use HCT116 and probably breast cancer cell lines.

09 February 2021 2,063 6 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View