I want to use the laser dissection function of the Zeiss PALM microscope. We have PEN membrane Petri dishes and cultured cells on them. When i laser the cells i can see the laser and also the shape that is lasered, but it is not catapulted into the cap. So is there anyone who has the same problem or knows what is going wrong?
Alliance Tony-Mayeko
Optical resolution of LCM is limited because sections become dried out owing to a lack of a coverslip, which is necessary for tissue capture (Fend et al., 2000). Commonly used stains, such as hematoxylin and eosin, are sometimes not effective for precise isolation of homogeneous cell populations
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