Hi Milad,
Micronuclei (MN) formation can in several ways. It might be due to oxidative DNA damage that shears off a chunk of the DNA from the chromosomes and without centromere, these chunk are left behind and form an extra-nuclear nuclei, which we term as MN. In another mechanism, it has been proposed that spindle motor protein dysfunction might cause a lagging segregation and prior to karyokinesis, the lagging strand fails to incorporate the nucleic material within the main nucleus, thus forming an extra-nuclear inclusion which we find as MN.
Based on your system, you have to interpret the observation or justify your work with experimentations. Hope this will help your cause.
Best,
Somnath.
the below link actually give you the best mechanism on Micronuclei formation and the possible related nuclear anomalies...
http://www.ncbi.nlm.nih.gov/pubmed/21164193
hope this would help you to understand the mechanisms well!
good luck
Hello
The link by Alex is just right for understanding micronucleus.
Your study involves fish, I guess you are looking at in vitro short term cultures of blood added with cytochalasin-B? Or are you looking at the bone marrow cells (like we do in mice - am not aware about fish model)? The mechanism will be clear from the above link. The frequency of MN in blood cells reflect level of exposure to genotoxic agents - this could be in vivo, or following addition to test compound in cultures; which is to be compared with control for statistical significance. Baseline level of MN is not expected to be 0 in controls, hence it will be increased frequency that will tell us something about the possible exposure.
Basically exposure of cells during G0 will menifest at next division, if not lethal to the cell in question.
Hope this helps, it was new for me to know about MN study in fish.
Best wishes
In situ quantification of MN in fish has been shown to be a suitable biomarker in assessing the impact of xenobiotics in aquatic ecosystems. The two species studied showed formation MN, with the greatest frequency A. manniorum and lowest in C. carpio. The mechanism formation of these abnormalities is not very clear. MN not associated in principle with body condition of the fish, phenomenon has also been observed in other species. A. manniorum exhibited increased sensitivity the potential presence of xenobióticos agents.
HI
this matter penetrate in The core and sits on a DNA string..
if you see in the comet assy test the broken strings
you seen that have arisen as a result of this matter
At the time of electrophoresis, in the test of comet cells, the cells are removed from the Glass slide and fall into the electrophoresis tank . What is the method that does not happen?
i would like to ask how to account micronucli in 2000 cells in fish blood?
Dear Milad,
I suggest you see this article:
Gamma radiation induced micronuclei and erythrocyte cellular abnormalities in the fish Catla catla. http://dx.doi.org/10.1016/j.aquatox.2012.06.001
Best regards
Dear Ragaa Fathy,
I suggest you see this article:
"The Micronucleus Assay in Fish Species as an Important Tool for Xenobiotic Exposure Risk Assessment—A Brief Review and an
Example Using Neotropical Fish Exposed To Methylmercury"
DOI: 10.1080/10641260903067852
Dear Daniel,
The problem is that most researches do not tell us how to count and calculate
Dear Ragaa Fathy,
In this article can see the count:
Micronucleus test in fishes as indicators of environmental quality in
subestuaries of the Río de la Plata (Uruguay) Marine Pollution Bulletin https://www.researchgate.net/deref/http%3A%2F%2Fdx.doi.org%2F10.1016%2Fj.marpolbul.2014.10.027
PLEASE HOW TO GET THIS RESEARCH..RESEARCHES ON SCIENCE DIRECT NOT
FREE.
Marine Pollution Bulletin. Vol. 91, Issue 2, 28 February 2015, Pages 518-523
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