Hello Everyone. I have been working in multiple modifications of flavonoids mainly glycosylation and methylation. We have recently set up an anti-cancer activity testing lab and we carry out MTT assays for several cancer cell lines. Recently we are interested to label active flavonoids and elucidate their binding model and signalling pathway inside the cancer cells. Anyone having experience in labeling technique please do let me know. Thank you.
Xiaoge Gao
After glycosylation, you can label them with the dye 5-DTAF, which can bind with the Hydroxyl groups,
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Niranjan Koirala
Hello Xiaoge. Thank you so much for your valuable answer. Could you kindly send me a protocol to track the signalling pathway how and where an anti cancer drug acts?
Thank you
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