I focus on aggressive lymphoma (Diffuse large B-cell Lymphoma, Burkitt Lymphoma).
By the variants I mean eg. an Epstein-Barr-Virus(EBV)-negative Burkitt cell line that has been in vitro transformed with EBV and both cell lines are available)
I don't know exactly any cell line, but we use the ECACC, AGTC, DSMZ cell banks. Check it out.
Hola Julie, you can see the ATCC web: http://www.atcc.org/ or another cell culture collection. In the ATCC for example, there are CRL-1596 cell line negative for Epstein-Barr virus. CCL-213 cell line carries Epstein-Barr virus. You can find more cell lines from lymphoma there.
Hey, thank you for answering! I have browsed the ATCC site already and found these cell lines too, but I was actually wondering if there are cell lines that are identical except for their EBV status (eg. the CRL-1596 which is EBV-negative and a CRL-1596 variant that has been in vitro infected with EBV). I would like to buy such cell lines because it would be difficult for me to infect eg CRL-1596 myself (because it requires working with active viral particles).
Some of the commercially available cell lines are not well-characterised and of dubious provenance. We can provide matched EBV-positive and negative BL cell lines if you are interested.
That would be great, thank you! Can I contact you about this via e-mail?
(And thank you too, Constantinos, it's always useful to know that!)
Dear Julie
There are two addresses:
(1) DSMZ cell banks, Germany. Email: [email protected]
(2) Oliver Schmetzer , MD; The Max Delbrück Centre for Molecular Medicine, 13125 Berlin. Phone +49 30 9406 0, Fax +49 30 949 4161. Oliver Schmetzer offered me
Burkitt-Lymphoma cell lines. Please ask him....
Good luck
best
eva
I suggest you use Mantle Cell Lymphoma cell lines (see the link) as EBV-negative lymphoma lines and Burkitt's lymphoma cell lines like RAJI as EBV-positive cell lines.
http://www.lifescienceglobal.com/home/cart?view=product&id=94
You cJIan use Mantle Cell Lymphoma as EBV-negative lymphoma lines RaJI as EBV-positive cell lines.
Yes, there are:
Raji cell line (EBV-positive, BL-derived); Ramos (EBV-negative, BL-derived); Akata cell line (EBV-positive), and others. All these lines coulb be obtained at the American Type Culture Collection, Manassas, VA or ATCC,Milan, Italy:
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