Can anyone tell the full form of BOX in BOX PCR?

Can anyone tell the full form of BOX in BOX PCR?

07 August 2018 8,349 1 View

What are the harmful impact of using DAP fertilizer on pigeon pea growth?

In India Di ammonium phosphate (DAP) is the most commonly used fertilizer for pigeon pea. But can someone tell me the authentic reports which proves that its causes harmful impact on the growth of...

04 May 2018 5,759 0 View

Can anyone tell me how probiotics can overcome the constraint of malnutrition in India ?

How can probiotic reduce the problem of malnutrition in developing country like India?

02 March 2016 7,513 3 View

An anyone tell me the simplest protocol for measuring leaf area?

Can anyone tell me the simplest protocol for measuring leaf area of arhar crop?

01 January 1970 2,884 1 View

How can we check confrontation assay between plant and bacteria without involving a pathogen?

Confrontation assay is usually conducted between a bacteria and plant pathogen but is there any method of checking this assay between plant and bacteria?

01 January 1970 8,849 1 View

Is there any universal primer for exopolysaccahride gene?

01 January 1970 4,377 7 View

Can anyone tell me the method of coextracting DNA and RNA simultanelusly from the bacterial sample?

There are different protocols for DNA and RNA extraction, but can anyone help me in telling the quickest and easiest method for extracting both (DNA and RNA) simultaneously from both gram positive...

01 January 1970 4,900 6 View

Can any one share method of sample preparation for LC -MS?

01 January 1970 8,310 20 View

How can we check Nod metabolite produced by rhizobium?

There are several methods for checking the presence or absence of Nod gene in Rhizobium. Like we can go for nodulation assay ( in tube,pot or field) or we can check the presence or absence of Nod...

01 January 1970 536 14 View

Can anyone tell me selective medium for isolating fusarium udum?

please tell me selective medium for isolating fusarium udum?

01 January 1970 7,251 8 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Genescan gives 3 peaks instead of one?

Hi all, I am doing a genescan analysis for a deleted exon in the patient sample. The amplified region is 215 bp. In the patient sample because of homozygous deletion, no peak is observed while in...

23 February 2021 5,645 1 View

QPCR reaction efficiency with odd results what does this indicate?

I am working on a CFX 96 machine to determine reaction efficiency of my gene of interest. After performing a serial dilution of my cDNA template, I was able to find great reaction efficiencies for...

23 February 2021 809 3 View