Can anyone tell the full form of BOX in BOX PCR?

Can anyone tell the full form of BOX in BOX PCR?

07 August 2018 8,349 1 View

What are the harmful impact of using DAP fertilizer on pigeon pea growth?

In India Di ammonium phosphate (DAP) is the most commonly used fertilizer for pigeon pea. But can someone tell me the authentic reports which proves that its causes harmful impact on the growth of...

04 May 2018 5,759 0 View

Can anyone tell me how probiotics can overcome the constraint of malnutrition in India ?

How can probiotic reduce the problem of malnutrition in developing country like India?

02 March 2016 7,513 3 View

Can anyone tell me genus specific primers of Bradyrhizobium?

Can anyone tell me genus specific primers of Bradyrhizobium.

01 January 1970 9,023 1 View

An anyone tell me the simplest protocol for measuring leaf area?

Can anyone tell me the simplest protocol for measuring leaf area of arhar crop?

01 January 1970 2,884 1 View

How can we check confrontation assay between plant and bacteria without involving a pathogen?

Confrontation assay is usually conducted between a bacteria and plant pathogen but is there any method of checking this assay between plant and bacteria?

01 January 1970 8,849 1 View

Is there any universal primer for exopolysaccahride gene?

01 January 1970 4,377 7 View

Can anyone tell me the method of coextracting DNA and RNA simultanelusly from the bacterial sample?

There are different protocols for DNA and RNA extraction, but can anyone help me in telling the quickest and easiest method for extracting both (DNA and RNA) simultaneously from both gram positive...

01 January 1970 4,900 6 View

Can any one share method of sample preparation for LC -MS?

01 January 1970 8,310 20 View

Can anyone tell me selective medium for isolating fusarium udum?

please tell me selective medium for isolating fusarium udum?

01 January 1970 7,251 8 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View