Can any one share method of sample preparation for LC -MS?

More Sakshi Tewari's questions See All

Can anyone tell the full form of BOX in BOX PCR?

07 August 2018 8,527 1 View

What are the harmful impact of using DAP fertilizer on pigeon pea growth?

In India Di ammonium phosphate (DAP) is the most commonly used fertilizer for pigeon pea. But can someone tell me the authentic reports which proves that its causes harmful impact on the growth of...

04 May 2018 5,961 0 View

Can anyone tell me how probiotics can overcome the constraint of malnutrition in India ?

How can probiotic reduce the problem of malnutrition in developing country like India?

02 March 2016 7,843 3 View

Can anyone tell me genus specific primers of Bradyrhizobium?

Can anyone tell me genus specific primers of Bradyrhizobium.

01 January 1970 9,200 1 View

An anyone tell me the simplest protocol for measuring leaf area?

Can anyone tell me the simplest protocol for measuring leaf area of arhar crop?

01 January 1970 3,078 1 View

Is there any universal primer for exopolysaccahride gene?

if anyone can help me in forwarding the papers in which universal q PCR primers for rhizobial eps gene are available. It will be a great help.

01 January 1970 4,557 7 View

How can we check confrontation assay between plant and bacteria without involving a pathogen?

Confrontation assay is usually conducted between a bacteria and plant pathogen but is there any method of checking this assay between plant and bacteria?

01 January 1970 9,037 1 View

Can anyone tell me the method of coextracting DNA and RNA simultanelusly from the bacterial sample?

There are different protocols for DNA and RNA extraction, but can anyone help me in telling the quickest and easiest method for extracting both (DNA and RNA) simultaneously from both gram positive...

01 January 1970 5,072 6 View

How can we check Nod metabolite produced by rhizobium?

There are several methods for checking the presence or absence of Nod gene in Rhizobium. Like we can go for nodulation assay ( in tube,pot or field) or we can check the presence or absence of Nod...

01 January 1970 718 14 View

Can anyone tell me selective medium for isolating fusarium udum?

please tell me selective medium for isolating fusarium udum?

01 January 1970 7,479 8 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

How can major oxides determined by ICP-MS/ES be normalized to 100% volatile free? How does this connect with LOI?

I am analyzing cherts from different sources found within one specific geologic formation using ICP-MS/ES. My committee was asking this question about my analysis. I was not sure how to answer this

05 August 2024 7,902 3 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

Usage of internal standards in LC-MS/MS analysis?

Have you ever seen a LC-MS/MS method uses both internal standards and external standards (in matrix matching purpose) but the concentrations of internal standards are outside the calibration curve...

05 August 2024 3,084 6 View

How can we use mobile apps for improving students' academic performance?

Mobile apps can be a powerful tool for enhancing academic performance, how can we use mobile apps for improving academic performance

04 August 2024 9,492 0 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

What are possible strategies can be used to analyze data under sequential explanatory mixed method approach?

Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches

04 August 2024 2,703 6 View

Why 3 replicates for most biological assays? Is it enough to examine the data fits normal distribution?

Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...

31 July 2024 8,141 13 View

What is the best practice for resuspending cell pellets during competent cell preparation (both electro and comp)?

I'm fairly new to making homemade comp cells and have done so with some mild success. What is generally an acceptable way of resuspending comp cells during wash steps? I've been told to swirl the...

31 July 2024 8,309 0 View

Laith Al-Ani

153

Hello Sakshi

I hope find many useful references on attachment 1

https://www.polyomics.gla.ac.uk/assets/downloads/MSMetabolomicsPrepCells-Aug2013.pdf

https://www.thermofisher.com/iq/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/sample-preparation-mass-spectrometry.html

https://www.mdpi.com/1422-0067/16/2/3537/pdf

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2905857/

https://www.mdpi.com/2218-1989/7/2/13/pdf

https://www.agilent.com/cs/library/primers/Public/5991-3326EN_SPHB.pdf

https://www.ag.arizona.edu/research/larkinslab/protocols/LC.MS.MS%20Sample%20Preparation.pdf

https://pubs.acs.org/doi/pdf/10.1021/ac981991q

https://pubs.rsc.org/en/content/articlehtml/2014/an/c4an00094c

http://www.gelifesciences.com/file_source/GELS/Service%20and%20Support/Documents%20and%20Downloads/Handbooks/Protein_sample_preparation_handbook.pdf

https://www.jbmethods.org/jbm/article/view/106/105

https://www.kumc.edu/mspc/sample-preparation.html

http://www.nature.com/nprot/journal/v11/n4/full/nprot.2016.042.html

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0160555

http://cf.gu.se/digitalAssets/1445/1445414_zeiss_labprotocol_proteinhandling_lcms_0611.pdf

https://sciex.com/Documents/tech%20notes/autoEthanol_Metabolites_in_Urine.pdf

https://www.technologynetworks.com/analysis/go/lc/view-application-note-231045

https://preomics.com/resources/preomics-ist-kit-protocol-v_2_5.pdf

http://www.biotech.cornell.edu/sites/default/files/uploads/Documents/Proteomics_protocols/Protocol%2016_iTRAQ%20labeling.pdf

http://www.beckman.com/getattachment/6b4a1aae-7e43-44a6-b148-46516fdce52e/Automated%20Sample%20Preparation%20for%20the%20Monitoring%20of%20Pharmaceutical%20and%20Illicit%20Drugs%20by%20LC-MSMS