What are the harmful impact of using DAP fertilizer on pigeon pea growth?

In India Di ammonium phosphate (DAP) is the most commonly used fertilizer for pigeon pea. But can someone tell me the authentic reports which proves that its causes harmful impact on the growth of...

04 May 2018 5,759 0 View

Can anyone tell me how probiotics can overcome the constraint of malnutrition in India ?

How can probiotic reduce the problem of malnutrition in developing country like India?

02 March 2016 7,513 3 View

Can anyone tell me genus specific primers of Bradyrhizobium?

Can anyone tell me genus specific primers of Bradyrhizobium.

01 January 1970 9,023 1 View

An anyone tell me the simplest protocol for measuring leaf area?

Can anyone tell me the simplest protocol for measuring leaf area of arhar crop?

01 January 1970 2,884 1 View

How can we check confrontation assay between plant and bacteria without involving a pathogen?

Confrontation assay is usually conducted between a bacteria and plant pathogen but is there any method of checking this assay between plant and bacteria?

01 January 1970 8,849 1 View

Is there any universal primer for exopolysaccahride gene?

01 January 1970 4,377 7 View

Can anyone tell me the method of coextracting DNA and RNA simultanelusly from the bacterial sample?

There are different protocols for DNA and RNA extraction, but can anyone help me in telling the quickest and easiest method for extracting both (DNA and RNA) simultaneously from both gram positive...

01 January 1970 4,900 6 View

Can any one share method of sample preparation for LC -MS?

01 January 1970 8,310 20 View

How can we check Nod metabolite produced by rhizobium?

There are several methods for checking the presence or absence of Nod gene in Rhizobium. Like we can go for nodulation assay ( in tube,pot or field) or we can check the presence or absence of Nod...

01 January 1970 536 14 View

Can anyone tell me selective medium for isolating fusarium udum?

please tell me selective medium for isolating fusarium udum?

01 January 1970 7,251 8 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View