Can anyone tell the full form of BOX in BOX PCR?
There is no full form of BOX. It is used here to highlight a repetitive sequence element generally a fixed 154 bp (box element) in bacterial genome.
In India Di ammonium phosphate (DAP) is the most commonly used fertilizer for pigeon pea. But can someone tell me the authentic reports which proves that its causes harmful impact on the growth of...
04 May 2018 5,961 0 View
How can probiotic reduce the problem of malnutrition in developing country like India?
02 March 2016 7,843 3 View
Can anyone tell me genus specific primers of Bradyrhizobium.
01 January 1970 9,200 1 View
Can anyone tell me the simplest protocol for measuring leaf area of arhar crop?
01 January 1970 3,078 1 View
if anyone can help me in forwarding the papers in which universal q PCR primers for rhizobial eps gene are available. It will be a great help.
01 January 1970 4,557 7 View
Confrontation assay is usually conducted between a bacteria and plant pathogen but is there any method of checking this assay between plant and bacteria?
01 January 1970 9,037 1 View
Please share protocol for sample preparation before performing LC-MS.
01 January 1970 8,507 20 View
There are different protocols for DNA and RNA extraction, but can anyone help me in telling the quickest and easiest method for extracting both (DNA and RNA) simultaneously from both gram positive...
01 January 1970 5,072 6 View
There are several methods for checking the presence or absence of Nod gene in Rhizobium. Like we can go for nodulation assay ( in tube,pot or field) or we can check the presence or absence of Nod...
01 January 1970 718 14 View
please tell me selective medium for isolating fusarium udum?
01 January 1970 7,479 8 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I have face this problem anyone help me how to solve this issue ?which is below Fatal error: There are inconsistent shifts over periodic boundaries in a molecule type consisting of 78 atoms. The...
07 August 2024 2,598 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...
05 August 2024 10,079 0 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Read the journal article by Douglas M. Lambert, “The Eight Essential Supply Chain Management Processes,” Supply Chain Management Review, Vol. 8, No. 6 (2004), pp. 18-26
04 August 2024 9,919 4 View
How does the atmosphere affect the flow of matter and energy on Earth and flow of energy in the biosphere related to the flow of food through a food chain?
02 August 2024 9,644 0 View