.Can anyone suggest some references about the use of WST-1 in plant cell culture?
Hi everyone, this topic is completely new to me, so i need some help to proceed with the easiest (if possible) method to achieve proteins extraction from strawberry fruits! Thanks in...
22 April 2024 2,609 0 View
As consultant and a researcher I am very eager to study family firms so to help them succeed, as I believe they are a very important element of the economic and social (family, community)...
15 April 2024 7,422 0 View
I am working on recycled cups made by Polystyrene and I am trying to dissolve them completely in Acetone and/or Ethyl Acetate solvent. However, it is quite hard to get it and I am not obtaining a...
25 March 2024 794 3 View
Hi everyone! I want to perform an immunofluorescent staining of spheroids from human glioblastoma cells fixed in 4% PFA. I found many protocols and papers suggesting different solutions and times...
02 March 2024 4,617 2 View
How can I improve viability when thawing PBMCs with 20% DMSO? Is there a protocol that can separate the dead cells from the viable ones?
07 February 2024 8,893 4 View
I would like to analyze mitochondria morphology (shape), and measure mitochondria length and number in Mitotracker-stained HDFs cells (40X and 60X images). Is there an easy-to-use tool or software...
18 March 2022 3,681 4 View
I would like to use the Geriatric Depression Scale to investigate the efficacy of a therapy treatment on depressive symptoms in people with dementia. I am aware of the cut-off and score range of...
28 February 2022 196 6 View
I know that 24, 48 and 72 are common but less than 24 hours is "allowed"? If my drug has some activity (low IC50) against some bacteria's strains in only 4 or 6 hours, is it enough to prove its...
26 January 2022 5,391 1 View
I have been using a t test to do it, considering homoscedastic and two-tailed t test (acceptable level of more than 70%), but I don't know if it is appropriate... Also, I don't know how much...
05 December 2021 8,630 2 View
I transfected my cells (primary human dermal fibroblasts) with a plasmid containing the gene of interest and a C-terminal turboGFP tag. I could see the turboGFP fluorescence using a fluorescence...
11 November 2021 7,272 3 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...
03 August 2024 9,918 0 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View