MAD or boxplot to detect outliers in non normally distributed data for latency and distance in animal cognition?

I am studying animal cognition and I have data about animals' latency to approach a cue and animals' distance from the cue in a test. Data are not normally distributed and when I do scatterplots...

04 February 2021 503 3 View

Someone has never seen a small band in control white lane not present in other near lanes?

In some end point PCR, I observ a weak band in white lane (no DNA) not present in any other lane with samples to assay and not according to the expected PCR product. I exclude a contamination of...

16 December 2020 4,011 5 View

Excel: How to maintain original conditional formatting of a cell when it is copied into a new location?

Hi! Question about conditional formatting in excel: I have a table in which in the first column there are species names and in the second column numeric values. I used graded colour scale...

12 August 2020 4,900 2 View

How to change the journal after manuscript acceptance?

Hi everyone, I have an issue and I'm hoping you can give me possible solutions or some advice. I, and two other co-authors, had an accepted paper (since 2019 October) in a great wildlife journal...

17 May 2020 5,513 16 View

What is the role of GABA in a protein purification protocol?

Dear all, I'm looking for Cpf1 purification protocol and I found a protocol in which the authors added GABA to the buffers ( from equilibration till the eluition). I'd like to know if I can...

03 May 2020 7,636 6 View

Which program is best to use for making phylogenetic trees (for beginners)?

Hi everyone, I am a Ph.D. student and work in the field of parasitology. I regularly use molecular techniques and would like to approach phylogenetic trees. More specifically, recently, I have...

08 March 2020 9,610 9 View

Site-directed mutagenesis with Superfi Master Mix, Does anyone use this kit?

08 May 2019 6,498 3 View

How long can DNA from dead parasites be detected in mammal samples by qPCR?

We have detected DNA from a protozoan parasite in lymph nodes of infected mice during 15 days after the infection. Could this DNA be related to live parasites?

04 June 2017 6,496 5 View

Ade4package for Mantel Test: Non convenient dimension In addition: Warning message: In as.dist.default(m2) : non-square matrix

22 May 2017 3,039 1 View

How does k63 ub change upon mg132 treatment?

Hi Guys, I cannot find anything in literature. I have olny found a paper in yeast.. I see that K63 ubiquitination decreases upon mg132 treatment. I would have expected an increase...any cues? Tnx...

03 May 2017 570 3 View

What is the biochemistry of acidic solvents for phenolics extraction in strawberry?

I am using the Folin-Ciocalteu method for analysis of total phenolic content in ripe strawberries. I've read several protocol that suggest a methanol/H2O/formic acid (80:19:1) extraction solution....

22 February 2021 6,560 3 View

How can I improve the concentration of banana fruits extracted RNA?

Currently, I'm working with banana fruits. I've tried to do RNA extractions with different methods, and I've gotten total RNA without contamination, but I couldn't get a high concentration of...

22 February 2021 6,126 2 View

Does calculating the interaction index between a drug and a food matrix make sense?

Dear all, I'm conducting the revisions of some of my manuscript, with is the impact of the combination of Aspirin and a fruit juice in colorectal cancer cells. Two of the reviewers have asked to...

16 February 2021 3,141 3 View

Why is there cloudy white solution in flavonoid test tube?

I do flavonoid test for biscuit incorporated with muntingia fruit powder. Sample extracted with metanol (1:10 ,w/v), macerated 24h, sentrifuged and filtered. Supernatan used for analysis. I have...

08 February 2021 6,779 4 View

Does the UV radiation affect on fruits ?

I want to examine a kind of fruit's extract's antimicrobial effects. At first I want to put it under the UV radiation to prevent the mold or any kind of fungi's and bacteria's growth. The...

24 January 2021 8,350 20 View

Can any one suggest the name of Litchi insect/predator?

Is it a type of Ambush bug or what? Thanks

14 January 2021 9,381 3 View

Can I make a meta-analysis with different variables in the same forest plot?

14 January 2021 1,733 4 View

How to determine the genetic potential of the genotype in terms of fruit size and weight?

In addition to genetic and environmental factors, fruit size is also influenced by crop load, rootstock, orchard planting system/tree training system, pollination, water and nutrient management,...

13 January 2021 9,309 4 View

What steps should one take to address post-harvest disease problems in a supply chain?

If I were to receive an import of a new fruit species in the country and, upon arrival, the whole lot was diseased, what steps do I take to diagnose and solve the problem? Is there a general...

10 January 2021 1,649 7 View

How we can isolate" distinguish" between Tyrophagus putrescentiae and T. entomophus ?

If I want to isolate Tyrophagus entomophagus by selective media, as I know they can grow on molds of stored grains,fruits, cheese. Or even in beehives debris. But how can I got only one species...

30 November 2020 9,087 3 View