MAD or boxplot to detect outliers in non normally distributed data for latency and distance in animal cognition?

I am studying animal cognition and I have data about animals' latency to approach a cue and animals' distance from the cue in a test. Data are not normally distributed and when I do scatterplots...

04 February 2021 503 3 View

Someone has never seen a small band in control white lane not present in other near lanes?

In some end point PCR, I observ a weak band in white lane (no DNA) not present in any other lane with samples to assay and not according to the expected PCR product. I exclude a contamination of...

16 December 2020 4,011 5 View

Excel: How to maintain original conditional formatting of a cell when it is copied into a new location?

Hi! Question about conditional formatting in excel: I have a table in which in the first column there are species names and in the second column numeric values. I used graded colour scale...

12 August 2020 4,900 2 View

How to change the journal after manuscript acceptance?

Hi everyone, I have an issue and I'm hoping you can give me possible solutions or some advice. I, and two other co-authors, had an accepted paper (since 2019 October) in a great wildlife journal...

17 May 2020 5,513 16 View

What is the role of GABA in a protein purification protocol?

Dear all, I'm looking for Cpf1 purification protocol and I found a protocol in which the authors added GABA to the buffers ( from equilibration till the eluition). I'd like to know if I can...

03 May 2020 7,636 6 View

Which program is best to use for making phylogenetic trees (for beginners)?

Hi everyone, I am a Ph.D. student and work in the field of parasitology. I regularly use molecular techniques and would like to approach phylogenetic trees. More specifically, recently, I have...

08 March 2020 9,610 9 View

Site-directed mutagenesis with Superfi Master Mix, Does anyone use this kit?

08 May 2019 6,498 3 View

How long can DNA from dead parasites be detected in mammal samples by qPCR?

We have detected DNA from a protozoan parasite in lymph nodes of infected mice during 15 days after the infection. Could this DNA be related to live parasites?

04 June 2017 6,496 5 View

Ade4package for Mantel Test: Non convenient dimension In addition: Warning message: In as.dist.default(m2) : non-square matrix

22 May 2017 3,039 1 View

How does k63 ub change upon mg132 treatment?

Hi Guys, I cannot find anything in literature. I have olny found a paper in yeast.. I see that K63 ubiquitination decreases upon mg132 treatment. I would have expected an increase...any cues? Tnx...

03 May 2017 570 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

How do I choose contrasts in SPM8?

I am learning how to use SPM for PET and PET-MR analysis, but I haven't found any great resources that explain how contrasts work. I have a series of PET images where our hypothesis argues that...

24 February 2021 1,534 2 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

What is the most prevalent drug related problem in the developed countries?

Detecting the drug related problems is an important step in the pharmaceutical care plan

21 February 2021 5,421 3 View

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19 February 2021 8,647 3 View