I have been using a t test to do it, considering homoscedastic and two-tailed t test (acceptable level of more than 70%), but I don't know if it is appropriate...
Also, I don't know how much concentrations should I use to do this comparison. I mean, I know that in common validation method I should use only 3 concentrations (5x each), but for this type method I don't know if is enough ou I should use all the 5 linearity concentrations (5x each).
For the matrix effect also 3 concentrations are enough?
I will be very grateful if someone could help with those questions...
Thanks in advance!
Hello, as I see from your description the for two tailed t test 3 concentration 5- times each. Not enough for linearity conformation. In this case better to use 5 concentration with 3 times each. (RSD
Thank you Karen.
Yes, I have been using 5 concentrations for the curve and so on, but for ME for example, as in a common validation, I have been using only three (low QC, medium QC and high QC). I just don't know if I can do it or if I should use all the concentrations tested...
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