Can two-photon absorption/excited fluorescence happen using continuous light source?

Guys, I have a question out of my curiosity about two-photon absorption/excitation. I know that the TPA probability is dependent on the square of light intensity. Pulsed laser is usually used as...

04 May 2018 8,941 1 View

If the absorbance spectra and excitation spectra don't overlap, what info can we extract from it?

Hi Guys, I need some info regarding UV-Vis spectrum and excitation spectrum. I understand excitation spectrum is scanning the excitation wavelengths by fixing one luminescence wavelength. And...

02 March 2018 9,317 5 View

How to separate proteins/polymers without color?

Hi Guys, I have a question regarding using Sephardex column to separate protein/ or PEI from unreacted small molecule. However, the small molecule does not have color. Is there method to tell when...

09 October 2016 1,033 2 View

How to renature proteins?

Guys, I have a question. How to re-nature the protein after the denature from GdmCl or Urea. Just curious! Thank you!

08 September 2016 9,261 8 View

Can Anyone Advise me on ompA signal peptide?

Hi guys, I am doing protein expression in BL21(DE3) and plan to use ompA signal for periplasmic protein translocation. I want to know that if it is OK to have a tag, say T7 or flag tag before ompA...

03 April 2016 5,613 3 View

Can anyone advise on IRES mRNA translation?

Hi guys, I plan to use IRES for consecutive reporter protein expression. Could anyone tell whether I should add the start codon to the piece of mRNA after the IRES sequence? Thank you!

01 February 2016 3,485 4 View

Can anyone adivse me on helper phage for phage display?

Guys, I want to understand more about the function about helper phage and I start reading. One articles says that 'When wild-type phages are used, interference of the plasmid with the phage...

01 February 2016 3,949 14 View

Can anyone advise me on a dual Luciferase assay using stable cell line and lentiviral transfection?

Hi Guys, Wish someone could help me out of this. Right now I am going to have Dual luciferase transfection (Firefly and Renilla) and I want to have stable cell line that can express both of the...

01 February 2016 7,246 2 View

How to determine the diversity of phage display?

I have just entered the training of phage display and now confused with the diversity determination. As far as I know, the first round of selection has higher diversity than the second and the...

31 December 2015 4,256 9 View

I got asymmetric peak in plamonic resonance in copper sulfide nanoparticle. Why?

I have verified the crystallinity and phase purity. EDX also tells me the particle belongs to covellite with Cu:S~ 1:1. So I have no clue in the asymmetric peak of its LSPR absorbance! I also have...

06 July 2014 5,520 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View