Can anyone advise on IRES mRNA translation?

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Syed A Ali

Did not quite get you. IRES is used for bicistronic expression i.e., two cistrons or genes are seperated by IRES element and expressed under the control of single promoter updtream of the 1st cistron. However, I will suggest you to consider 2A peptide. Cistron downstream IRES tends to express poorly.

Maixian Liu

Thanks Ali. Maybe I should put in this way. See the patter

Cistron1----IRES----Cistron2.

Does Cistron2 need a start codon for translation into protein?

Thank you