Maixian Liu

11 Questions 14 Answers 0 Followers

Questions related from Maixian Liu

Can two-photon absorption/excited fluorescence happen using continuous light source?

Guys, I have a question out of my curiosity about two-photon absorption/excitation. I know that the TPA probability is dependent on the square of light intensity. Pulsed laser is usually used as...

05 May 2018 8,986 1 View

If the absorbance spectra and excitation spectra don't overlap, what info can we extract from it?

Hi Guys, I need some info regarding UV-Vis spectrum and excitation spectrum. I understand excitation spectrum is scanning the excitation wavelengths by fixing one luminescence wavelength. And...

03 March 2018 9,385 5 View

Can anyone suggest on using plvx-based lentivirus from clonetech for renilla luciferase transfection?

Hi Guys, I cloned the Renilla luciferase (hRluc from promega) into the vector of plvx from clone tech (puromycin resistance). I have tried 3 times of transfecting MDA-MB-231 cells for stably...

11 November 2016 9,691 0 View

How to separate proteins/polymers without color?

Hi Guys, I have a question regarding using Sephardex column to separate protein/ or PEI from unreacted small molecule. However, the small molecule does not have color. Is there method to tell when...

10 October 2016 1,074 2 View

How to renature proteins?

Guys, I have a question. How to re-nature the protein after the denature from GdmCl or Urea. Just curious! Thank you!

09 September 2016 9,307 8 View

Can Anyone Advise me on ompA signal peptide?

Hi guys, I am doing protein expression in BL21(DE3) and plan to use ompA signal for periplasmic protein translocation. I want to know that if it is OK to have a tag, say T7 or flag tag before ompA...

04 April 2016 5,679 3 View

Can anyone advise on IRES mRNA translation?

Hi guys, I plan to use IRES for consecutive reporter protein expression. Could anyone tell whether I should add the start codon to the piece of mRNA after the IRES sequence? Thank you!

02 February 2016 3,533 4 View

Can anyone advise me on a dual Luciferase assay using stable cell line and lentiviral transfection?

Hi Guys, Wish someone could help me out of this. Right now I am going to have Dual luciferase transfection (Firefly and Renilla) and I want to have stable cell line that can express both of the...

02 February 2016 7,297 2 View

Can anyone adivse me on helper phage for phage display?

Guys, I want to understand more about the function about helper phage and I start reading. One articles says that 'When wild-type phages are used, interference of the plasmid with the phage...

02 February 2016 3,992 14 View

How to determine the diversity of phage display?

I have just entered the training of phage display and now confused with the diversity determination. As far as I know, the first round of selection has higher diversity than the second and the...

01 January 2016 4,329 9 View

I got asymmetric peak in plamonic resonance in copper sulfide nanoparticle. Why?

I have verified the crystallinity and phase purity. EDX also tells me the particle belongs to covellite with Cu:S~ 1:1. So I have no clue in the asymmetric peak of its LSPR absorbance! I also have...

07 July 2014 5,562 4 View