Can two-photon absorption/excited fluorescence happen using continuous light source?

Guys, I have a question out of my curiosity about two-photon absorption/excitation. I know that the TPA probability is dependent on the square of light intensity. Pulsed laser is usually used as...

04 May 2018 8,941 1 View

If the absorbance spectra and excitation spectra don't overlap, what info can we extract from it?

Hi Guys, I need some info regarding UV-Vis spectrum and excitation spectrum. I understand excitation spectrum is scanning the excitation wavelengths by fixing one luminescence wavelength. And...

02 March 2018 9,317 5 View

Can anyone suggest on using plvx-based lentivirus from clonetech for renilla luciferase transfection?

Hi Guys, I cloned the Renilla luciferase (hRluc from promega) into the vector of plvx from clone tech (puromycin resistance). I have tried 3 times of transfecting MDA-MB-231 cells for stably...

10 November 2016 9,649 0 View

How to separate proteins/polymers without color?

Hi Guys, I have a question regarding using Sephardex column to separate protein/ or PEI from unreacted small molecule. However, the small molecule does not have color. Is there method to tell when...

09 October 2016 1,033 2 View

How to renature proteins?

Guys, I have a question. How to re-nature the protein after the denature from GdmCl or Urea. Just curious! Thank you!

08 September 2016 9,261 8 View

Can Anyone Advise me on ompA signal peptide?

Hi guys, I am doing protein expression in BL21(DE3) and plan to use ompA signal for periplasmic protein translocation. I want to know that if it is OK to have a tag, say T7 or flag tag before ompA...

03 April 2016 5,613 3 View

Can anyone advise on IRES mRNA translation?

Hi guys, I plan to use IRES for consecutive reporter protein expression. Could anyone tell whether I should add the start codon to the piece of mRNA after the IRES sequence? Thank you!

01 February 2016 3,485 4 View

Can anyone adivse me on helper phage for phage display?

Guys, I want to understand more about the function about helper phage and I start reading. One articles says that 'When wild-type phages are used, interference of the plasmid with the phage...

01 February 2016 3,949 14 View

How to determine the diversity of phage display?

I have just entered the training of phage display and now confused with the diversity determination. As far as I know, the first round of selection has higher diversity than the second and the...

31 December 2015 4,256 9 View

I got asymmetric peak in plamonic resonance in copper sulfide nanoparticle. Why?

I have verified the crystallinity and phase purity. EDX also tells me the particle belongs to covellite with Cu:S~ 1:1. So I have no clue in the asymmetric peak of its LSPR absorbance! I also have...

06 July 2014 5,520 4 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

No title

02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View