Hi, i am trying to induce apoptosis in A549 cells and MDA MB 231 cells. I use etoposide for A549 cells and TRAIL for MDA MB 231 cells. I usually detect apoptosis induction by Annexin V staining immunofluorescence. If i treat more, cell detaches from 4 well chamber slide, if less then no induction of apoptosis.

Can anyone suggest me the concentration, time of treatment, FBS percentage and effective annexin v staining protocol to get annexin v positive cells under immunofluorescence.

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