Md. Enamul Haque

6 Questions 7 Answers 0 Followers

Questions related from Md. Enamul Haque

How to detect streptavidin magnetic bead- biotinylated protein conjugation ?

I am using M270 strepatividin magnetic bead to conjugate with biotinylated protein or RNA. How can i detect that my biotinylated protein or RNA is conjugated with streptavidin magnetic beads ?

11 March 2021 1,513 2 View

Having blue smear during M13 phage titering, any idea?

I am using M13 phage peptide library for biapanning of specific peptide. After first 2 rounds of screening, i am now observing a blue layer on agar plate. I have diluted the phage upto highest...

16 September 2019 7,747 3 View

How to subculture Tumorsphere Culture of MDA MB 231 Breast Cancer Cell Lines ?

I am trying to generate sphere formed MDA MB 231 cells using Mammocult media from Stem Cells. After 7 days of culture in ultra low adherent plate, the formed tumorsphere. When i tried to filter...

11 December 2017 4,474 0 View

Renal carcinoma xenograft model with 769-P renal adenocarcinoma cell line?

I am working with a Peptide that targets renal carcinoma. I have checked the binding with 769-P cells in vitro. Now i want to develop an in vivo tumor model using 769-P cells. But most the...

31 August 2016 838 3 View

Can anyone suggest me effective concentration of Etoposide for A549 lung carcinoma cells and TRAIL concentration for MDA MB 231 cell apoptosis

Hi, i am trying to induce apoptosis in A549 cells and MDA MB 231 cells. I use etoposide for A549 cells and TRAIL for MDA MB 231 cells. I usually detect apoptosis induction by Annexin V staining...

14 April 2016 4,602 2 View

Why is DAPI unable to penetrate the cell membrane but Hoechst stain does?

DAPI is usually used to stain fixed/permeabilized cells whereas Hoechst stain can be used to stain live cells also.

16 January 2014 736 5 View