What is the best suited direct protocol for miRNA isolation from exsosome?

I want to isolate exosomal miRNA only, for that do I need to first isolate total RNA and then go for miRNA isolation? or can I directly isolate miRNA from exosome and do the analysis? if yes...

08 December 2020 5,803 4 View

For electrochemical capacitors which type of specific capacitance is more technically preferable to represent?

Capacitance of electrochemical capacitors generally represented in relative scale, i.e. the units are represented either related to electrode area or the mass of active material. In most of the...

22 June 2019 8,243 5 View

What are the usefulness of Support Vector Machine Learning?

What are the usefulness of Support Vector Machine Learning?

03 May 2018 1,312 3 View

Why Id/Ig ratio increases after reduction of Graphene oxide and Ad/Ag ratio reduces for the same ?

Most of the time I have observed that Id/Ig ratio increases after reduction of GO and and Ad/Ag ratio decreases [where A denotes area under the peak]. Could you please help me by sharing your...

30 January 2018 7,220 5 View

Is it possible to use agilent 4156c semiconductor parameter analyzer as potentiostat for three electrode electrochemical measurement ?

I want to use agilent 4156c SPA as potentiostat for three electrode electrochemical measurement. It is possible to specify different scan rates (V/Sec) and potential windows (V) by this...

07 November 2017 7,421 2 View

Is it possible to find a Eulerian path or Eulerian cycle in polynomial time in a directed multigraph with self loops?

20 September 2014 2,498 2 View

No title

Using Gaussian 09

18 May 2014 7,786 2 View

rectal length

what is the correct length of the human rectum?

17 March 2010 5,178 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Can I put a figure that was drawn by Bio-Render in publication paper?

I want to publish paper in journal but I have figures which have drawn with bio-renders, can I put them in the paper or which other software I can use to draw them?

27 February 2021 8,179 1 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

How the values of errors can be calculated for thermodynamic data?

I have calculated thermodynamic parameters for surfactant solutions in aqueous and mixed alcoholic solvents. I want to calculate the value of errors in the data and represent it in the table along...

22 February 2021 239 2 View

How to visualise pathways from a phosphoproteomics dataset?

I am looking for something similar than Ingenuity Pathway Analysis (IPA) in which I can enter the proteins differentially phosphorylated and I can visualise the pathways these proteins are...

21 February 2021 8,923 4 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

Recommendations for sample size in visual qualitative research (using children's drawings)?

Hi everyone! Asking for a little help, if I may. I am working on a research project, where I aim to analyse children's drawings about Covid-19. I am fairly new to using visual data in qualitative...

07 February 2021 9,530 3 View

What is the best way to fix plasma derived miRNA for subsequent cDNA synthesis?

I need to quantify blood plasma derived miRNA through q-RT-PCR. For this I need to fix RNA content in my cDNA reaction. What is the best way to do this? Currently, I take readings of my plasma...

03 February 2021 9,330 3 View