Hi ,
There are a lot of protocols but most of them requires culture these cells as a part of the technique , for my project i need easy protocol to preparation and analysis hMSC of placenta tissues without culture cells ( mean work direct on tissue)
Best Regards
Hi Saleh,
For Placenta tissue cells you actually get many other cells as well. So to get the population of MSCs you will have to culture them on plastic, which is one of the characteristic step for obtaining more number of cells. Otherwise you can go for FACS sorting for MSC specific markers but then it should be done under sterile conditions.
I agree with Pankaj Mogha. FACS Sorting is your best option. You have to select one or two population before hands in order to buy labeling markers whenever you intend to do sorting. And frankly it will be expensive if you choose to sort and isolate 4 or more of different cell types. In this case culture will be better off for you.
Hello
Thank you very much P. Mogha & B.M. Alrefaei , for these recommendations. It is appreciated.
Good Luck
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