If a journal doesn't provide doi after acceptance of the manuscript, is it authentic?

It is related to submission of the manuscript in a Journal and the process of its online availability.

18 February 2021 9,269 5 View

How can AI be used for enabling Design for Sustainability?

Design for Sustainability (DfS) is a wicked and complex problem. It needs the designer to combine specialized knowledge from several disciplines with systemic thinking. The designer also needs to...

05 February 2021 2,998 9 View

Des anybody have a straight forward protocol for invasion assay using colon cancer stem cells? Is there necessary to dilute the Matrigel?

I am working on colon cancer stem cells to see the impact of combinational therapy.

23 December 2020 9,830 3 View

Is there any software (windows) or spreadsheet except MELTS to understand fractional crystallization behaviour of volcanic rocks (i.e. basalts)?

I have bulk rock major and trace elements data available to me.

17 November 2020 3,531 3 View

Have anyone used galaxy server ?

I have 4 fastq files. From some earlier post I tried to analysethrough Galaxy sever. I used "fastp", "clip adapter" and also "trimomatic" separately three different times to trim adapter. As I...

05 November 2020 1,589 1 View

How to scale an image whose scaling details are unknown?

I want to know how to add scale bar to a histopathological image using ImageJ whose image scale was not recorded at the time of taking the image.I got to know from the technicians that 1micron is...

03 November 2020 10,009 3 View

How to do scoring for fat droplets in Histopathological sections?

I want to do scoring for macrovesicles and microvesiclular fat diposits in Histopathological Liver sections. Can anyone suggest me a software and discuss a suitable protocol to achieve this. I...

18 October 2020 9,727 2 View

Significant value post Bonferroni correction?

Hi I did Friedman's RM ANOVA and found data is significant.Then Wilcoxon posthoc analysis is showing significant differences, p=0.001.But Bonferroni adjusted significance is bringing p value to...

07 October 2020 1,262 3 View

How to remove background and gridlines of graph in R?

I am trying to remove the background & gridlines from my Violin plot drawn by R. Please let me know the script/method. Also please let me know method to move the axis title n graph title in R....

07 October 2020 391 4 View

How to underatand whether to consider Linear, Quadratic or Cubic regression equation?

Hi I want to understand the relationship between two variables using correlation and regression. I performed Normality test and my data is parametric.After doing scatter plot analysis I observed...

29 September 2020 9,533 10 View

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02 March 2021 3,060 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

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22 February 2021 9,932 11 View