Im trying to establish a co culture platform for N2A and glial glial cell line for neurodegenerative disorder study..
I see some papers have directly cocultured cell lines.. but I need a system where I can control the cells seperately but have them in same environment.
I would appreciate any suggestions.
Thank you
There are cell culture inserts fitting into different plates for this - this is the routine way, i think. Another simple method (i never tried, but read about) to add one of the lines on coverslip to the other; or you can use a cloning cylinder to isolate first the lines than see what happens without the cylinder (cells are on the same coverslip/dish in this case. for longer studies, they can mixed surely without cylinder).
Standard method would be Boyden chamber, see link.
Another approach is to fluorescently label different cell populations with different fluorescent dyes, just like in our attached paper.
There are also co-culture tissue culture plastic, see the link for example.
http://ibidi.com/xtproducts/en/ibidi-Labware/Open-Slides-Dishes:-ibidi-Standard-Bottom/m-Slide-2-x-9-Well
https://www.emdmillipore.com/US/en/life-science-research/antibodies-assays/antibodies-overview/Research-Areas/cancer/metastasis-cell-invasion/boyden-chamber-technique/I0qb.qB.KSMAAAFANtY.1ZcQ,nav
Article Interaction and self-organization of human mesenchymal stem ...
I used inserts to co-culture astrocytes and microglia. It is hard to scratch cells in the small insert. If you want to do RNA extraction I recommend the big ones.
Best regards
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