Can anyone suggest for best method for ROS content measurement in florescence microscope of filamentous fungi ?

28 May 2022 0 3K Report

The protocol that I used:

20 microMole of DCHF-DA in PBS saline.

I took Ganoderma fungal mycelia on a glass slide from the broth-grown medium in a petri dish with and without copper stress, washed in PBS buffer and teased with a needle, and stained for 30 minutes at 30 degree celsius temperature.

After visualization in the microscope slide was completely green so the mycelia did not appear clearly.

Kindly suggest me for better visualization of ROS content in mycelia of Ganoderma lucidum.

Thank you

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