Can anyone suggest an optimized protocol for collagen degradation/digestion using collagenase enzyme?

20 November 2023 1 6K Report

I need information on concentration of enzyme to be used for particular amount of collagen, the buffer solutions, temperature, time, pH required for the optimal enzyme activity.

According to the papers I referred, I used 0.05M Tris-HCl and 5mM CaCl2 solution for enzyme activity but didn't seem to workout.

Paola Campomenosi

Hi Siddhi,

You should be more specific in describing what is your starting material, what type of analysis you want to do, what is your aim, otherwise it is difficult to suggest the correct procedure.

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