I have DH5alfa and wild type E. coli strains. Which strain I should use for the non-capsulated control? Will there be a total absence of capsule?
E.coli is not a capsulated organism.
For capsulated organism-you should use Aerobacter aerogenes or Klebsiella pneumoniae, Pnemococci, Leuconostoc strains. - some examples.
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Pig slurry is rich in major and minor nutrients. Is there any way to improve / Enrich its manure quality to be used in agriculture organically ? please share your knowledge.
09 August 2024 5,605 2 View
Hi, I'm currently working on a project where I need to plot the atom-projected band structure using GPAW. I've been able to calculate the band structure for my material, but I'm having trouble...
07 August 2024 269 3 View
Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...
07 August 2024 3,218 4 View
Hi How can this equation Ln(LA) = 1.038 + 0.89 ln(X) be applied to calculate the leaf area of a tomato? Can you explain with an example and what is the substitution of Ln and ln?
06 August 2024 2,508 2 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...
05 August 2024 9,805 3 View
XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds
04 August 2024 3,019 3 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Machine learning (ML) has shown great potential in predicting the compressive strength of concrete, an important property for structural engineering. However, its practical application comes with...
03 August 2024 2,546 2 View
I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...
04 August 2024 5,228 4 View
NA
11 July 2024 8,969 1 View
Hello everyone. Could you help me with the following problem. I am going to determine ascorbic acid in capsules using an AOAC method. The method gives the following equation: mg ascorbic...
24 June 2024 789 1 View
For the estimation of carbohydrates there are different methods. As D39 serotypes contain glucuronic acid as one of the monosaccharide sugars, napthoresorcinol can be used to quantify it. if...
17 June 2024 888 0 View
I am planning to perform a phagocytosis assay for THP1-derived M2 macrophages. According to the Vybrant phagocytosis assay kit, it says to incubate the cells in the assay plate for an hour before...
10 January 2024 538 1 View
I am having trouble losing small specimens while washing and changing solutions in 1.8 ml cryo tubes (after high pressure freezing). Does anyone know of something to help keep the samples at the...
13 December 2023 4,273 0 View
We all know that the absorption of fluconazole is unaffected by food or gastric PH....... So, what's the effect of emptying the content of fluconazole capsule in glass of water on drug afficacy???
12 November 2023 1,328 0 View
Hi all. I'm interested in a possibly trivial question. What is the real significance of pH 4.5 and 6.8 dissolution media when analyzing solid dosage forms when we have immediate release in pH 1.2...
02 November 2023 1,368 1 View
capsule/ fresh/dry and or...
13 October 2023 3,266 1 View
I synthesized microcapsules and then centrifuged at 500rpm with water and later rinsed with absolute ethanol. Then, I vacuum filtered and spread the capsules on a filter paper with spatula, placed...
28 September 2023 9,425 1 View