I am a novice in the field of biochemistry. Thus, need inputs on what current feasible biochemical assays are used to elucidate the role of an enzyme in a biological pathway.
I supose enzymatic assays. Maybe try different assays for speed and durability as in microarrays, screening,(metabolomics, proteomics? ). I think you might need several methods and not just one. Maybe start by searching substrates or ligands