Is it reasonable to use half your dataset to carry out exploratory analysis leading to specific hypotheses, and then test these using the other half?

There is a clear distinction between exploratory and confirmatory research. In exploratory research, we explore our data to discover interesting trends or associations, perhaps by analysing it in...

25 July 2019 9,588 4 View

How to add published research directly from the publication web site?

I have published articles and a PhD thesis which are not listed here. How can I add the link to the publication site here?

17 May 2019 4,398 2 View

What is the experience with Duck Duck Go as a search engine?

Concern about data mining and the uses to which the mined data are put suggest that privacy is not just a matter of personal sensitivities. It may, at least partially, be a social duty if it can...

15 September 2018 6,988 4 View

How does taking multiple measurements under the same condition affect experiment power and effect sizes?

In our research group we carry out psychophysics experiments that involve taking multiple measurements, e.g. reaction times or correct detections, under the same combination of variables. I...

01 August 2018 7,372 7 View

Is there a source to learn which cell lines bind RGD (are integrin-expressing)?

Hello, I was wondering if anyone knows of a resource which lists cell lines (common ATCC.org ones) that bind RGD (and ones that don't). Ultimately, we'd like to obtain a cell line that has high...

25 March 2018 8,053 9 View

Looking for help building a layered structure in FDTD

I'm looking to build a layered quarter wave stack structure in a FDTD simulation. I've tried both 2-D and 3-D and it hasn't worked. I'm able to do a block of solid material but not layers. The...

10 August 2017 6,385 13 View

Is it appropriate to use a weighted sample size in calculating standard errors and conducting significance tests?

SPSS' regression procedure, when conducting a weighted analysis (to provide unbiased estimates of population parameters) bases standard errors and significance tests on the weighted N. For a...

02 January 2017 4,722 4 View

I'm trying to model choices over time. Does SPSS have a statistical procedure for this analysis?

This administrative study investigates the choices students make in a menu of choices curriculum design. Given a gateway course, where do they go from there? And then from there, etc.? I will have...

10 September 2015 4,881 7 View

How can I use an Instron for soil penetration resistance measurements?

12 June 2015 6,962 3 View

How to view output of dsr.cc?

hi all I did copy dsr.cc to scratch(/home/student/ns-allinone-3.21/ns-3.21/scratch) and renamed it to dsrtest.cc I did ./waf build build finished successfully I did ./waf --run...

23 February 2015 7,455 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View