HI Maulik,
I use the RNA extraction kit from Epicentre. Check this page: http://www.epibio.com/products/nucleic-acid-purification-extraction-kits/dna-purification-genomic/masterpure-complete-dna-and-rna-purification-kit
It's easy, cheap and a quick protocol. Downstream applications like RT-PCR etc. work perfectly.
All the best,
Nadine
If you are looking for economical method, then RNA isolation by manual method (Trizol method) would just be fine. However, in your case, you seem to extract RNA from tissue samples, then in that case, instead of storing your sample in liquid nitrogen you could your tissue samples in RNA later solution (whose recipe would be available in internet) and then store it in -80 degree deep freezer. It is very very economical and the yield will be absolutely fine.Hope it works
Hey Maulik, You can go for the Quick-RNA™ MiniPrep (RNA isolation kit) from Zymoresearch.
Even I use this and regularly I'm getting good results.
I can also recommend the Trizol method. Normally it works with all kinds of tissues and the yield of RNA you will get out of it is very high. If you need an protocol let me know.
You can also store your samples in Trizol at -80. What I do with my samples is to put them in Trizol freeze it with liquid nitrogen and store it than at -80.
Thank you all for answers. Commercial Kit is the best solution but for my study i want to go with economical methods without any commercial kits, that's why i ask, because mostly all the methods having LN use.
Thank you very much Srividya Subramanian .. for your suggestion. Can we use guanidine thiocyanate + sarkosyl in place of TRIzol..? Means i dont know its sufficient for RNA preparation as like TRIzol or not?
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