Pandit Ramesh

6 Questions 21 Answers 0 Followers

Questions related from Pandit Ramesh

How can I assemble paired end and shotgun sequencing data for whole genome assembly using SOAPdenovo2?

We have sequenced whole genome of fungi using 3K (250bp), 8K (250bp) and shotgun (450bp) library. How can we assemble these data up to scaffold level using SOAPdenovo2? Is there any good assembler?

09 September 2014 2,872 3 View

What is a good way to perform blast analysis of data against host pathogen interaction database? How can the HPI database be accessed?

I am working on Transcriptome sequencing of fungi. I would like to find pathogenecity related genes from fungi. HPI database provides this information, but I do not know how to make a blast or...

08 August 2013 1,778 5 View

For how long are the expressed mRNA stable in fungi?

I have to perform an induction experiment. If i preserve my induced fungal culture at -20C then could it affect the level of expressed genes? For how much time I can preserve it? Or do I have to...

02 February 2013 5,264 3 View

I have sequenced a protease encoding gene from my fungi. How can I learn more about the coding regions and the presence of introns or exons?

I have sequenced about a 700 bp gene fragment of protease from genomic DNA. Now I would like to know the coding regions and whether it has any introns? Which software should I use?

02 February 2013 4,870 5 View

Hello friends i am working on Nematophagous fungi. Please anybudy give me name of book or paper or anything from which i can get classification of nem...

Generally it classification is based on morphlogy of fungi especially its traping structure and size and shape of spores.

12 December 2011 7,946 0 View

PLZ any one suggest me some free software through i can design specific primers for only Real Time PCR?

I would like to study expressed genes diring fungi infection.

12 December 2011 7,062 0 View