Can anyone suggest a protocol for protoplast transformation for the transformation of Bacillus subtilis wild?

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Can we preserve the protoplasts?

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Nikunj Daga

I have a document. if you could share an id. I will forward it to you.

Daniel R Zeigler

A substantial number of wild Bacillus subtilis strains can be transformed by the method of Anagnostopoulos and Spizizen (PMID: 16561900). Most can be transformed with plasmid DNA by the method of Xue et al 1999 J. Microbiol. Meth. 34:183–191. You might also want to consider the method of Meddeb-Mouelhi et al (PMID: 22387342). I have attached links and some PDF files describing my implementation of these methods.

http://www.ncbi.nlm.nih.gov/pubmed/16561900

http://www.ncbi.nlm.nih.gov/pubmed/22387342

Wiep Klaas Smits

Are you refering to transformation with replicative plasmid, non-replicating plasmids or chromosomal DNA?

Otávio Bravim

Hello!! I have been working with other stuff lately and there is a long time I'm outta here. I appreciate the answers. I'm really sorry for how long it took me to view them.

Nikunj Daga, I'll send you my email!

Daniel R Zeigler, I've tried some of these with no results, but numbers 2 and 3 I'll try as soon as possible, thanks!

Wiep Klaas Smits, I'm trying to tranform with a linearized integrative DNA, do you have any suggestions?