Wiep Klaas Smits

4 Questions 21 Answers 0 Followers

Questions related from Wiep Klaas Smits

What causes problem with MC1061-DH5a plasmid compatibility?

We are frequently using plasmids with a certain backbone. Due to circumstances, some of these were maintained in Ecoli MC1061 and others in DH5a. Recently, we experienced problems with digests...

24 March 2016 4,818 3 View

Does anyone know of an estimate about ug total protein per OD600=1 of bacterial cells?

I am trying to find out if there are any reasonable guestimates out there on the amount of protein in a total bacterial lysate from a certain amount of cells. All I could find so far is estimates...

15 February 2016 6,252 5 View

How can I deal with different rRNA depletion results in RNAseq?

Hi, We have conducted a bacterial RNAseq using rRNA depletion on a NextSeq. Upon analysis, it seems some samples still have a significant amount of reads (5-40%) mapping to rRNA genes. How does...

20 September 2015 1,780 4 View

Is there anybody using the rRNA depletion kit for RNAseq?

Was informed by service provider that a NGS project is put on hold pending backorder to Illumina's RiboZero bacterial rRNA depletion kit. Looking for somebody who can either still has that kit, or...

19 August 2015 6,862 4 View